Pig free thyroxine (FT4) ELISA kit

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產品詳情

產品描述：

豬游離甲狀腺素(FT4)酶聯免疫試劑盒（CSB-EQF027512PI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的FT4含量。FT4即游離甲狀腺素，是甲狀腺功能檢查重要指標。它可直接反映甲狀腺功能狀態。研究機制方面，FT4參與機體代謝調節，其水平變化與甲狀腺疾病如甲亢、甲減密切相關，檢測它有助于準確診斷和病情判斷。試劑盒檢測范圍為4 pmol/L-64 pmol/L，靈敏度為1.08 pmol/L。科研人員可將其應用于動物營養代謝研究、環境應激評估、甲狀腺相關疾病模型構建等領域，尤其適用于需要動態監測甲狀腺功能變化的實驗設計，如激素分泌調控、代謝性疾病機制研究或毒理學評價等，為畜牧獸醫研究及生物醫學基礎科研提供標準化檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

FT4
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

4 pmol/L-64 pmol/L
靈敏度：

1.08 pmol/L
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	95
	Range %	87-106
1:4	Average %	96
	Range %	90-110
1:8	Average %	91
	Range %	86-98

回收率：

The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-103
EDTA plasma (n=4)	96	93-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進展：

FT4指的是游離甲狀腺素，是指在血液中不與蛋白質結合的甲狀腺素。最新研究發現，FT4水平與代謝綜合征、糖尿病、心血管疾病等疾病密切相關。一項針對14,107名美國成年人的研究表明，FT4水平與冠狀動脈鈣化風險呈正相關關系。此外，一些研究表明，孕期FT4水平異常與胎兒神經發育相關的基因表達水平有關，提示FT4水平對胎兒神經發育的影響。