Pig complement fragment 5a (C5a) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬補(bǔ)體片斷5a(C5a)酶聯(lián)免疫試劑盒（CSB-EQ027637PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C5a含量。C5a是重要的免疫相關(guān)靶點(diǎn)。在補(bǔ)體系統(tǒng)激活過程中被裂解產(chǎn)生，具有趨化炎癥細(xì)胞、調(diào)節(jié)免疫反應(yīng)等作用。其異常激活與多種炎癥性疾病及自身免疫病相關(guān)，目前科研聚焦于研究其信號通路機(jī)制，以開發(fā)針對性治療藥物。試劑盒檢測范圍為9.375 ng/mL-600 ng/mL，適用于科研領(lǐng)域?qū)ωi源樣本中C5a水平的動(dòng)態(tài)監(jiān)測，例如免疫相關(guān)疾病模型研究、感染或炎癥狀態(tài)下補(bǔ)體系統(tǒng)激活程度的評估，以及藥物或疫苗開發(fā)中免疫調(diào)節(jié)效應(yīng)的體外檢測，為探索補(bǔ)體介導(dǎo)的病理生理機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

C5a
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

9.375 ng/mL-600 ng/mL
靈敏度：

2.34 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	91-109
1:2	Average %	87
	Range %	82-93
1:4	Average %	102
	Range %	98-106
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of pig C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	92-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.