Pig activated protein C(APC) ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬活化蛋白C(APC)酶聯免疫試劑盒（CSB-E12633p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APC含量。APC（腺瘤性息肉病基因）是一種腫瘤抑制基因，其突變與結直腸癌等癌癥的發生密切相關。研究機制顯示，APC基因突變會導致β-catenin蛋白積累，進而激活下游原癌基因，促進腫瘤形成。通過研究APC基因及其相關信號通路，有助于開發新的癌癥治療策略。試劑盒檢測范圍為40 pg/mL-8000 pg/mL，該產品適用于科研領域，如豬凝血機制研究、炎癥相關疾病模型（如敗血癥或血栓性疾病）的建立與評估，以及抗凝藥物或生物制劑開發過程中藥效動力學研究。其兼容多種樣本類型的特點，可支持不同實驗設計需求，例如通過組織勻漿分析特定器官（如肝臟或血管內皮）中APC的局部表達水平，為深入探究APC在病理生理過程中的調控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

APC
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

40 pg/mL-8000 pg/mL
靈敏度：

20 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	95
	Range %	89-101
1:4	Average %	94
	Range %	91-99
1:8	Average %	96
	Range %	93-99

回收率：

The recovery of pig APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	91-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.