Pig Thyroid Stimulating Hormone,TSH ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬促甲狀腺素(TSH)酶聯免疫試劑盒（CSB-E13719p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的TSH含量。TSH即促甲狀腺激素，是腺垂體分泌的促進甲狀腺生長和機能的激素。其主要作用是調節甲狀腺細胞的增殖和甲狀腺血液供應，調控甲狀腺細胞合成與釋放甲狀腺激素。在科研中，常圍繞其與甲狀腺疾病關系等機制展開研究，以助力疾病診斷與治療。試劑盒檢測范圍為0.6 μIU/mL-24 μIU/mL，適用于動物生理學研究、藥物開發中的甲狀腺功能評估、畜牧養殖健康監測等場景，例如探究環境應激對甲狀腺軸的影響、飼料添加劑或激素干預的效果分析等本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
縮寫：

TSH
Uniprot No.：

P01224
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

0.6 μIU/mL-24 μIU/mL
靈敏度：

0.3 μIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	93
	Range %	88-97
1:4	Average %	102
	Range %	98-106
1:8	Average %	88
	Range %	82-94

回收率：

The recovery of pig TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	102	98-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.