Pig Stromal cell derived factor 1a,SDF-1a ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬基質細胞衍生因子1a(SDF-1a/CXCL12)酶聯免疫試劑盒（CSB-E12055p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的SDF1a/CXCL12含量。SDF1a/CXCL12 是重要靶點。它作為趨化因子，在細胞遷移、增殖等生理過程起關鍵作用。在研究機制上，其通過與受體結合，激活下游信號通路。在腫瘤、炎癥等疾病中也有異常表達和功能，是藥物研發潛在方向。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于體外培養細胞模型、動物實驗組織樣本或生物體液中的SDF - 1a動態監測；為探索其在心血管疾病、再生醫學或免疫微環境調控中的功能機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

SDF1a/CXCL12
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell Lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	92
	Range %	88-96
1:4	Average %	86
	Range %	82-90
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of pig SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	86	82-90

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.