Pig Carboxypeptidase B(CPB1) ELISA kit

產(chǎn)品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬羧肽酶B(CPB1)酶聯(lián)免疫試劑盒（CSB-EL005883PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CPB1含量。CPB1，全稱羧肽酶B1，是一種胰腺特異性蛋白，作為急性胰腺炎和胰腺移植排斥反應(yīng)的血清標(biāo)志物。研究顯示，CPB1在胰腺癌全切術(shù)后復(fù)發(fā)和/或轉(zhuǎn)移中表達(dá)上調(diào)，與疾病進(jìn)展密切相關(guān)，可作為診斷和治療靶點(diǎn)。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于科研領(lǐng)域?qū)ωi模型消化系統(tǒng)功能、營養(yǎng)代謝調(diào)控及相關(guān)疾病機(jī)制的探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CPB1 ELISA Kit; CPBCarboxypeptidase B ELISA Kit; EC 3.4.17.2 ELISA Kit
縮寫：

CPB1
Uniprot No.：

P09955
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	92
	Range %	85-100
1:4	Average %	94
	Range %	86-102
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of pig CPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	95	90-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

基因功能參考文獻(xiàn)：
1. Its catalytically important Tyr248 residue is oriented and hydrogen-bonded to solvent water molecules, and locates the furthest away from the catalytic zinc ion as compared to previous structures. PMID: 19802820
亞細(xì)胞定位：

Secreted. Zymogen granule lumen.
蛋白家族：

Peptidase M14 family
數(shù)據(jù)庫鏈接：

KEGG: ssc:397341

STRING: 9823.ENSSSCP00000012464

UniGene: Ssc.16048