Mouse tumor necrosis factor α (TNF-α) antibody ELISA Kit

產品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠腫瘤壞死因子α(TNF-α)抗體酶聯免疫試劑盒（CSB-EQ023955MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的TNF-α Ab含量。TNF-α Ab即腫瘤壞死因子-α單克隆抗體，TNF-α在多種炎癥疾病中過度表達。TNF-α Ab的研究機制是其能特異性結合TNF-α，阻斷它與受體相互作用，減少炎癥信號傳導。它為類風濕關節炎等炎性疾病治療帶來新方向。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于炎癥機制研究、免疫治療藥物體外效果評估、感染性疾病模型分析等科研場景，為研究感染性休克、類風濕性關節炎等TNF-α相關疾病的分子機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

antibody for DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
縮寫：

TNF-α Ab
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	97
	Range %	90-105
1:4	Average %	87-96
	Range %	82-96
1:8	Average %	93
	Range %	88-100

回收率：

The recovery of mouse TNF-α antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.