Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit

Mouse tumor necrosis factor-like ligand 1 (TL1) ELISA kit

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中文名稱：

小鼠腫瘤壞死因子配體相關分子-1(TL1)酶聯免疫試劑盒
貨號：

CSB-E16311m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

小鼠腫瘤壞死因子配體相關分子-1(TL1)酶聯免疫試劑盒（CSB-E16311m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNFSF15含量。TNFSF15，也稱為TL1A，是一種腫瘤壞死因子超家族成員，主要在免疫細胞中表達。其通過與DR3受體結合激活信號通路，參與炎癥反應、細胞凋亡和腫瘤血管生成。研究顯示，TNFSF15在多種疾病如炎癥性腸病和腫瘤中扮演重要角色。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于科研實驗中TL1蛋白表達水平的動態監測，例如炎癥機制研究、免疫調控模型構建或藥物干預效果評估等領域。通過標準曲線定量分析，研究者可精確測定不同生理或病理狀態下樣本中TL1的濃度變化，為探索該分子在免疫相關疾病中的作用提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Tnfsf15 ELISA Kit; Tl1 ELISA Kit; Vegi ELISA Kit; Tumor necrosis factor ligand superfamily member 15 ELISA Kit; TNF ligand-related molecule 1 ELISA Kit; Vascular endothelial cell growth inhibitor ELISA Kit
縮寫：

TNFSF15
Uniprot No.：

Q5UBV8
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	104
	Range %	100-108

回收率：

The recovery of mouse TL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-93
EDTA plasma (n=4)	94	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.871	2.641	2.756	2.601
2000	2.086	2.067	2.077	1.922
1000	1.348	1.325	1.337	1.182
500	0.811	0.826	0.819	0.664
250	0.505	0.544	0.525	0.370
125	0.293	0.299	0.296	0.141
62.5	0.219	0.235	0.227	0.072
0	0.154	0.156	0.155

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

T cell expression of IL-18R and DR3 is essential for non-cognate stimulation of Th1 cells and optimal clearance of intracellular bacteria Pham OH.et al,PLoS Pathog,2017
Tumor necrosis factor superfamily member 15 (Tnfsf15) facilitates lymphangiogenesis via upregulation of Vegfr3 gene expression in lymphatic endothelial cells Qin TT. et al,J Pathol,2015
S100A9: A Potential Biomarker for the Progression of Non-Alcoholic Fatty Liver Disease and the Diagnosis of Non-Alcoholic Steatohepatitis Liu X. et al,PLoS One,2015

功能：

Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Promotes splenocyte alloactivation.
基因功能參考文獻：
1. TL1A blocking ameliorates intestinal fibrosis in the T cell transfer model of chronic colitis PMID: 29254800
2. ATF3 protects against LPS-induced acute lung injury by inhibiting TL1A expression. PMID: 28177121
3. this study shows that TL1A-/- mice are more susceptible to dextran sodium sulfate colitis PMID: 27233964
4. These data demonstrated a direct role for TL1A-DR3 signaling in tissue fibrosis and that modulation of TL1A-DR3 signaling could inhibit gut fibrosis. PMID: 24850426
5. TL1A deficiency impacts on the gut microbial composition and the mucosal immune system, especially the intraepithelial TCRgammadelta(+) T-cell subset, and that TL1A is involved in the establishment of adipose tissue. PMID: 25404161
6. Our data demonstrate a key role for TL1A in promoting ILC2s at mucosal barriers. PMID: 24220298
7. delivers survival signals to plasma cells and promotes pathogenic Ab production in collagen arthritis PMID: 24140642
8. TNFSF15 inhibits vasculogenesis by regulating relative levels of membrane-bound and soluble isoforms of VEGF receptor 1. PMID: 23918400
9. These results establish a novel link between tumor necrosis factor (TNF)-family cytokine TL1A (TNFSF15) and interleukin 13 (IL-13) responses that results in small intestinal inflammation. PMID: 20980995
10. role of TL1A in mucosal T cells and antigen presenting cells function and showed that up-regulation of TL1A expression can promote mucosal inflammation and gut fibrosis. PMID: 21264313
11. findings suggest that the transcription factor NF-kappaB plays an important role in the regulation of VEGI expression PMID: 15702971
12. neonatal CD4+ CD3- cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient PMID: 16920944
13. VEGI may have a key role in both the termination of angiogenesis and facilitation of dendritic cell maturation, which is the initial step in immune responses toward inflammatory stimuli. PMID: 17785811
14. TL1A may contribute to renal inflammation and injury through DR3-mediated activation of NF-kappaB and caspase-3 PMID: 18287561
15. Results reveal that TL1A is an important modulator in the development of chronic mucosal inflammation by enhancing T(H)1 and T(H)17 effector functions. PMID: 18598698
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亞細胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

KEGG: mmu:326623

STRING: 10090.ENSMUSP00000050144

UniGene: Mm.208152

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.