Mouse tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠基質金屬蛋白酶抑制因子2(TIMP-2)酶聯免疫試劑盒（CSB-E07387m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的TIMP2含量。TIMP2 是組織金屬蛋白酶抑制因子 2，在體內能抑制金屬蛋白酶活性，維持細胞外基質穩態。其研究機制主要聚焦于它在腫瘤發生、發展中作用，如抑制腫瘤細胞侵襲、轉移，還參與血管生成調控，有望成為癌癥等疾病治療新靶點。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于體外細胞實驗、動物模型研究及組織樣本分析，為探究TIMP-2在炎癥反應、腫瘤微環境調控或器官纖維化等領域的分子機制提供可靠工具，適用于分子生物學、病理機制研究等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
縮寫：

TIMP2
Uniprot No.：

P25785
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

125 pg/mL-8000 pg/mL
靈敏度：

31.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	106
	Range %	101-113
1:40	Average %	84
	Range %	80-88
1:80	Average %	95
	Range %	90-100
1:160	Average %	98
	Range %	94-105

回收率：

The recovery of mouse TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	88	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
亞細胞定位：

Secreted.
蛋白家族：

Protease inhibitor I35 (TIMP) family
組織特異性：

Detected in testis, retina, hippocampus and cerebral cortex.
數據庫鏈接：

STRING: 10090.ENSMUSP00000017610

UniGene: Mm.206505