Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit

說明書下載

產品詳情

產品描述：

小鼠可溶性肌球蛋白重鏈1(sMHC-1)酶聯免疫試劑盒（CSB-E11155m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MYH1含量。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，為研究肌肉相關疾病模型（如心肌缺血、骨骼肌萎縮等）提供可靠工具；科研人員可通過該產品分析運動生理學中肌肉適應性變化、評估藥物或基因干預對肌肉功能的影響，或探索肌肉退行性疾病的分子機制；適用于基礎研究、藥效評價及生物標志物篩選等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Myh1 ELISA Kit; Myosin-1 ELISA Kit; Myosin heavy chain 1 ELISA Kit; Myosin heavy chain 2x ELISA Kit; MyHC-2x ELISA Kit; Myosin heavy chain ELISA Kit; skeletal muscle ELISA Kit; adult 1 ELISA Kit
縮寫：

MYH1
Uniprot No.：

Q5SX40
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sMHC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	102
	Range %	97-106
1:4	Average %	85
	Range %	81-90
1:8	Average %	104
	Range %	100-108

回收率：

The recovery of mouse sMHC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	87	92-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Muscle contraction.
基因功能參考文獻：
1. Data suggest that single muscle immobilization induces a shift of myosin heavy chain (MHC) isoforms composition toward a faster contractile phenotype and decreases the polymorphic profile of single fibres, and that activation of p38 and JNK could be a potential mechanism involved in these contractile phenotype modifications during muscle immobilization. PMID: 27383612
2. p38alpha/beta MAPKs-mediated coactivator recruitment at a proximal MEF-2 site is important for MyHCIId/x gene regulation in skeletal muscle. PMID: 17210568
3. The maintenance of the relative expression of slow MHC-1 protein is independent of neuromuscular activity in mice. PMID: 18945940
亞細胞定位：

Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
蛋白家族：

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
數據庫鏈接：

KEGG: mmu:17879

STRING: 10090.ENSMUSP00000018637

UniGene: Mm.477065