Mouse pyridinoline (PYD) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠膠原吡啶交聯(lián)(PYD)酶聯(lián)免疫試劑盒（CSB-E15010m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的PYD含量。小鼠膠原吡啶交聯(lián)（PYD）是一種通過吡啶交聯(lián)劑對膠原蛋白進(jìn)行交聯(lián)的技術(shù)，旨在改善膠原蛋白的物理和生物性能。該技術(shù)通過交聯(lián)反應(yīng)增加膠原蛋白的穩(wěn)定性和機(jī)械強(qiáng)度，廣泛應(yīng)用于組織工程和再生醫(yī)學(xué)領(lǐng)域，以促進(jìn)組織修復(fù)和再生。試劑盒檢測范圍為50 ng/mL-800 ng/mL，適用于骨代謝機(jī)制研究、骨質(zhì)疏松動物模型評估、抗骨吸收藥物開發(fā)等科研領(lǐng)域，為骨代謝相關(guān)基礎(chǔ)研究提供高效可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PYD
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

50 ng/mL-800 ng/mL
靈敏度：

50 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	100
	Range %	92-106
1:4	Average %	85
	Range %	80-90
1:8	Average %	91
	Range %	83-99

回收率：

The recovery of mouse PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-97
EDTA plasma (n=4)	93	91-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.