Mouse pancreatic alpha amylase (AMY2A)ELISA kit

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產品詳情

產品描述：

小鼠胰α淀粉酶(AMY2A)酶聯免疫試劑盒（CSB-EL001689MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的AMY2A含量。AMY2A是一種與淀粉酶相關的靶點。它在人體消化過程中發揮作用，能將淀粉分解為糖類。研究機制上，聚焦于其基因表達調控、酶活性影響因素等，探索其與消化系統疾病的關聯，有望為相關疾病的診斷和治療提供新方向。試劑盒檢測范圍為9.38 mU/mL-600 mU/mL，適用于體外培養細胞模型、動物實驗樣本的酶活性評估，以及代謝干預或病理狀態下AMY2A動態變化的機制研究，為探索胰腺功能調控及消化系統相關疾病提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
縮寫：

AMY2A
Uniprot No.：

P00688
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

9.38 mU/mL-600 mU/mL
靈敏度：

2.3 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	92
	Range %	88-96
1:4	Average %	86
	Range %	80-92
1:8	Average %	89
	Range %	84-97

回收率：

The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-97
EDTA plasma (n=4)	101	94-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.