Mouse neutrophil elastase,NE ELISA Kit

Mouse neutrophil elastase,NE ELISA Kit

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文獻引用 (8) 評價 (1) 說明書下載

中文名稱：

小鼠中性粒細胞彈性蛋白酶(NE)酶聯免疫試劑盒
貨號：

CSB-E04804m
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠中性粒細胞彈性蛋白酶(NE)酶聯免疫試劑盒（CSB-E04804m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的ELA2含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，炎癥機制研究、疾病模型構建、藥物干預效果評估、肺損傷模型、自身免疫性疾病研究、腫瘤相關微環境分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Elane ELISA Kit; Ela2Neutrophil elastase ELISA Kit; EC 3.4.21.37 ELISA Kit; Elastase-2 ELISA Kit; Leukocyte elastase ELISA Kit
縮寫：

ELA2
Uniprot No.：

Q3UP87
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	88
	Range %	82-95
1:20	Average %	97
	Range %	93-101
1:40	Average %	99
	Range %	95-106
1:80	Average %	93
	Range %	87-99

回收率：

The recovery of mouse NE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	99	95-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.849	2.793	2.821	2.627
10	2.132	2.222	2.177	1.983
5	1.465	1.388	1.427	1.233
2.5	0.948	0.929	0.939	0.745
1.25	0.643	0.666	0.655	0.461
0.625	0.472	0.442	0.457	0.263
0.312	0.278	0.288	0.283	0.089
0	0.198	0.189	0.194

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Lead-induced actin polymerization aggravates neutrophil extracellular trap formation and contributes to vascular inflammation Q Nong, Y Wu, S Liu, Y Tang, J Wu, H Huang,Ecotoxicology and Environmental Safety,2025
Anti-inflammatory effect and pharmacokinetics of dehydroandrographolide, an active component of Andrographis paniculata, on Poly(I:C)-induced acute lung injury YG Liu,Biomedicine & pharmacotherapy,2024
Saponin extracts from Gynostemma pentaphyllum (Thunb.) Makino display sedative-hypnotic and anxiolytic effects CY Shen,Industrial Crops and Products,2020
Carbon black nanoparticles induce cell necrosis through lysosomal membrane permeabilization and cause subsequent inflammatory response X Yuan,Theranostics,2020
Effects of Cetyltrimethylammonium Bromide on the Toxicity of Gold Nanorods Both In Vitro and In Vivo: Molecular Origin of Cytotoxicity and Inflammation YP Jia,Small Methods,2020
Loss of complex O-glycosylation impairs exocrine pancreatic function and induces MODY8-like diabetes in mice Wolters-Eisfeld G.et al,experimental and molecular medicine,2018
Cigarette smoke promotes COPD by activating platelet-activating factor receptor and inducing neutrophil autophagic death in mice Lv XX.et al,Oncotarget,2017
Long-term azithromycin ameliorates not only airway inflammation but also remodeling in a murine model of chronic asthma Kang JY. et al,Pulm Pharmacol Ther,2016

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平均分:

5.0分 - 1 個評價

樣品類型：組織勻漿液肺組織勻漿上清

樣品信息：小鼠

稀釋比：沒有稀釋

產品評價：我通過使用CSB-E04804m檢測了PR8病毒感染的小鼠肺組織勻漿上清，操作簡單方便易上手，靈敏度非常高，檢測結果十分精準

By 鄧老師

功能：

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Capable of killing E.coli; probably digests outer membrane protein A (ompA) in E.coli.
基因功能參考文獻：
1. the impact of NE mutations that are typical of human congenital neutropenia in a murine model of in vitro and in vivo neutrophil differentiation PMID: 27942017
2. Levels of neutrophil elastase (NE) and the nuclear factors CCAAT/enhancer-binding protein alpha (C/EBPalpha) and growth factor-independent 1 (GFI-1) are elevated in hematopoietic stem and progenitor cells from high-fat diet (HFD)-fed mice, but mice lacking either NE or C/EBPalpha are resistant to HFD-induced myelopoiesis. PMID: 28202548
3. Sivelestat treatment significantly reduced the protein level, neutrophil count, neutrophil elastase activity in bronchoalveolar lavage fluid (BALF). PMID: 28300553
4. Serine protease required for resistance to microbial infection; contributes to pathogenessis in endotoxic shock PMID: 10714686
5. Neutrophils from neutrophil elastase-deficient mice show no defect in transendothelial migration PMID: 11994507
6. results of ultrasound studies using the elastase and AngII apoE in 4 biomechanical parameters: aneurysm diameter, aneurysm volume, circumferential cyclic strain, and blood flow velocity PMID: 26064906
7. Sirtinol is a novel HNE inhibitor and may have the potential for clinical application in the treatment of inflammatory lung diseases. PMID: 25666548
8. Increased ELANE activity was associated with development of autoimmune diabetes mellitus in NOD mice. PMID: 25092677
9. Neutrophil elastase is involved in the induction of microbicidal activities of neutrophils against Trypanosoma cruzi-infected macrophages. PMID: 24599360
10. contributes to clearance of Aspergillus fumigatus infections PMID: 24054721
11. In conclusion, sivelestat reduced radiationinduced lung injury in the mice by suppressing neutrophil elastase activity and excessive inflammatory reactions PMID: 23404144
12. Lung cancer promotion by inflammation is partly mediated by activation of the IL-8/CXCR2 pathway and subsequent recruitment of neutrophils and release of neutrophil elastase. PMID: 24321240
13. a neutrophil elastase inhibitor prevents bleomycin-induced pulmonary fibrosis in mice PMID: 22441751
14. During Pseudomonas aeruginosa infection, interleukin (IL)-1beta processing is dependent on neutrophil elastase and not NLRC4 or caspase-1. PMID: 23024281
15. Neutrophil elastase modulates cytokine expression: contribution to host defense against Pseudomonas aeruginosa-induced pneumonia. PMID: 22927440
16. NADPH oxidase in antimicrobial host defense against A. fumigatus and B. cepacia, whereas the proteases neutrophil elastase, cathepsin G, and lysosomal cysteine protease cathepsin C/ dipeptidyl peptidase I are dispensable PMID: 22163282
17. Cathepsin G and neutrophil elastase contribute to lung-protective immunity against mycobacterial infections in mice. PMID: 22461690
18. Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. PMID: 21979170
19. This is the first study showing a contribution of neutrophil-derived neutral serine proteases CG and NE to lung-protective immunity against focal pneumonia-inducing serotype 19 S. pneumoniae in mice. PMID: 21911460
20. The selective sensitivity of G913X Elane granulocytic cells to ER stress provides new and strong support for the unfolded protein response model of disease pathogenesis in severe congenital neutropenia. PMID: 21285438
21. neutrophil infiltration and neutrophil elastase (NE) activity are elevated in the chronic UVB-irradiated skin of hairless mice, and confirmed the involvement of NE in MMP activation PMID: 20965700
22. In the absence of a pathogen challenge, neutrophil-derived proteases and nucleosomes contribute to large vessel thrombosis, the main trigger of myocardial infarction and stroke. PMID: 20676107
23. Since NE is maximally produced in promyelocytes, this protease may play a role in APL pathogenesis by facilitating the leukemogenic potential of PML-RARalpha. PMID: 14636558
24. neutrophil elastase and cathepsin G are inhibited by PAI-1 mutants PMID: 15131125
25. Neutrophil elastase has a role in PML-retinoic acid receptor alpha activities in early myeloid cells PMID: 15601827
26. When challenged intraperitoneally, mice deficient in neutrophil elastase are markedly more susceptible to Klebsiella infection and death. PMID: 15661916
27. PECAM-1, alpha6 integrins and neutrophil elastase work together to mediate neutrophil transmigration PMID: 15840647
28. The results of the present study strongly suggest that neutrophil elastase may represent a common pathogenic link between emphysema and fibrosis. PMID: 16045796
29. Neutrophil elastase depends on serglycin proteoglycan for localization in granules. PMID: 17272511
30. This study describes for the first time a novel hierarchy among proteases whereby a serine protease NE up-regulates expression of MMPs and cathepsins. PMID: 17442971
31. Neutrophil elastase and proteinase 3 complexes have different interaction patterns with peptidic substrates. PMID: 18023421
32. Neutrophil elastase cleaves laminin-332 (laminin-5) generating peptides that are chemotactic for neutrophils. PMID: 18178964
33. required for neutrophil egression from the vasculature into the alveolar space; interfering with this process leads to neutrophil-related endothelial cell injury. PMID: 18276796
34. neutrophil elastase might decrease the pulmonary endothelial production of prostacyclin by inhibiting endothelial NO production PMID: 18323746
35. PR3 and neutrophil elastase enhance neutrophil-dependent inflammation by eliminating the local antiinflammatory activity of progranulin. Our results support the use of serine protease inhibitors as antiinflammatory agents. PMID: 18568075
36. Neutrophil elastase (NE) degrades the major outer membrane protein F (OmpF); a targeted deficiency in NE negates its antibacterial role in vitro and in a Pseudomonas aeruginosa pneumonia model. PMID: 18802098
37. during acute peritoneal inflammation, NE expression and activity increase gradually, facilitating leukocyte influx. PMID: 19088179
38. Evidence for a role of Ela2 in antimicrobial immunity and endotoxic shock PMID: 10714686
39. Evidence for a role of neutrophil elastase in bleomycin-induced fibrosis PMID: 11451907
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蛋白家族：

Peptidase S1 family, Elastase subfamily
數據庫鏈接：

KEGG: mmu:50701

STRING: 10090.ENSMUSP00000038925

UniGene: Mm.262194

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.