Mouse mitochondrial respiratory chain complex I ELISA kit

文獻引用 (1) 說明書下載

產品詳情

產品描述：

小鼠線粒體呼吸鏈復合物I酶聯免疫試劑盒（CSB-EQ027280MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的MRCC-I含量。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，本試劑盒采用高特異性配對抗體，通過標準化的預包被板與顯色系統實現目標蛋白的定量分析，適用于基礎研究中線粒體功能評估、疾病模型構建（如心肌缺血、阿爾茨海默癥等）或藥物干預后能量代謝變化的動態監測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

MRCC-I
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MRCC-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-100
1:2	Average %	100
	Range %	94-103
1:4	Average %	94
	Range %	84-98
1:8	Average %	100
	Range %	93-103

回收率：

The recovery of mouse MRCC-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-97
EDTA plasma (n=4)	99	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.