Mouse copeptin ELISA kit
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中文名稱:小鼠和肽素(copeptin)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E14921m
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠和肽素(copeptin)酶聯(lián)免疫試劑盒(CSB-E14921m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的copeptin含量。copeptin是精氨酸加壓素原的C端片段,穩(wěn)定性好,可反映精氨酸加壓素的釋放水平。在研究機(jī)制上,它可作為生物標(biāo)志物用于評(píng)估多種疾病。如在心血管疾病、腎臟疾病等的診斷、病情監(jiān)測(cè)及預(yù)后評(píng)估方面展現(xiàn)出潛在價(jià)值。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL,適用于基礎(chǔ)科研中探討小鼠模型在應(yīng)激性疾病、水鹽代謝異常、心血管功能障礙或神經(jīng)內(nèi)分泌紊亂等生理病理過程中的分子機(jī)制,為動(dòng)物實(shí)驗(yàn)提供高效的分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:copeptin
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:3.12 pg/mL-200 pg/mL
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靈敏度:0.78 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-98 1:2 Average % 96 Range % 92-102 1:4 Average % 102 Range % 96-108 1:8 Average % 94 Range % 89-99 -
回收率:
The recovery of mouse copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 87-98 EDTA plasma (n=4) 88 82-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 200 2.145 2.044 2.095 1.943 100 1.744 1.643 1.694 1.542 50 1.113 1.092 1.103 0.951 25 0.683 0.672 0.678 0.526 12.5 0.361 0.372 0.367 0.215 6.25 0.294 0.283 0.289 0.137 3.12 0.236 0.232 0.234 0.082 0 0.151 0.152 0.152 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Role of Fructose and Fructokinase in Acute Dehydration Induced Vasopressin Gene Expression and Secretion in Mice. Song Z.et al,J Neurophysiol. ,2016
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