Mouse coagulation factor Ⅴ,FⅤ ELISA Kit

Mouse coagulation factor Ⅴ,FⅤ ELISA Kit

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中文名稱：

小鼠凝血因子Ⅴ(FⅤ)酶聯免疫試劑盒
貨號：

CSB-E17815m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

小鼠凝血因子Ⅴ(FⅤ)酶聯免疫試劑盒（CSB-E17815m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的F5含量。F5即凝血因子Ⅴ，作為凝血過程關鍵因子，在凝血共同途徑中起加速凝血酶原向凝血酶轉化作用。其基因突變會導致易栓癥或出血性疾病等。研究聚焦其結構功能、與疾病關聯機制，為相關疾病診斷治療提供理論支撐。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，適用于基礎科研中探究凝血機制、評估藥物或基因干預對凝血功能的影響，以及構建凝血相關疾病模型（如遺傳性凝血缺陷或血栓性疾病）時監測FⅤ的表達水平變化。為血液學、藥理學及心血管疾病研究提供可靠工具，實驗結果僅用于科研分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

F5Coagulation factor V ELISA Kit; Activated protein C cofactor) [Cleaved into: Coagulation factor V heavy chain; Coagulation factor V light chain] ELISA Kit
縮寫：

FⅤ
Uniprot No.：

O88783
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

78 ng/mL-5000 ng/mL
靈敏度：

19.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Blood Coagulation
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅤ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-103
1:200	Average %	100
	Range %	95-104
1:400	Average %	95
	Range %	90-100
1:800	Average %	98
	Range %	94-105

回收率：

The recovery of mouse FⅤ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	88	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5000	2.351	2.521	2.436	2.240
2500	1.823	1.907	1.865	1.669
1250	1.354	1.294	1.324	1.128
625	0.853	0.893	0.873	0.677
312	0.612	0.607	0.610	0.414
156	0.401	0.421	0.411	0.215
78	0.298	0.287	0.293	0.097
0	0.200	0.192	0.196

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
基因功能參考文獻：
1. Mice with the FVL mutation do not have increased spermatogenesis as compared to wildtype mice. PMID: 28927238
2. Platelet-derived FV is a critical mediator of arterial thrombosis that involves platelet activation. PMID: 28673898
3. Platelet-derived FV contributes to the control of angiogenesis and is likely associated with thrombin generation in hind limb ischemia model. PMID: 27237401
4. Mice deficient in LMAN1 exhibit FV and FVIII deficiencies and liver accumulation of alpha1-antitrypsin. PMID: 21795745
5. The FVL mutation does not influence coagulation activation, lung inflammation or survival in lethal influenza A. PMID: 20413539
6. It suggested that there could be a combination of GLA deficiency and FVL or other thrombosis-related gene defect in patients with genetic severe early-onset thrombosis. PMID: 20533258
7. that the murine platelet FV compartment is derived exclusively from primary biosynthesis within cells of marrow origin and an intact platelet FV pool is not required for protection from spontaneous hemorrhage or bleeding following minor trauma PMID: 12816857
8. the murine platelet and plasma FV pools are biosynthetically distinct PMID: 12855561
9. The source of the FVL leading to accelerated thrombosis appears to be circulating, non-platelet-derived plasma FVL. PMID: 15809370
10. FVL has the ability to improve the hemophilia A or B phenotype, but this effect is principally evident at the microcirculation level following a particular vascular injury. PMID: 16359510
11. observations demonstrate a synergistic interaction between alpha-galactosidase A deficiency and Factor V Leiden toward tissue fibrin deposition; concomitant mutations in these genes may increase the penetrance of vascular thrombotic events in humans PMID: 16514103
12. Fetal gene defects precipitate platelet-mediated pregnancy failure in factor V Leiden mothers. PMID: 17438064
13. The FVL mutation has no effect on the development of secondary tumours of colon cancer in livers of mice. PMID: 17635646
14. FV Leiden allele has no effect in murine colitis and we thus question the importance of activated blood coagulation in the etiology or pathogenesis of inflammatory bowel disease. PMID: 17849058
15. the factor V Leiden mutation is associated with enhanced thrombotic tendency after FeCl3 injury of the femoral artery on a standard fat diet, but not a high fat diet PMID: 17938812
16. In contrast to humans, pregnancy induced a decrease in APC resistance in wild-type and in FV Leiden mice. PMID: 19036061
17. Homozygous Factor V could promote atherosclerosis and fibrin deposition in apolipoprotein E deficient mice. PMID: 19671356
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亞細胞定位：

Secreted.
蛋白家族：

Multicopper oxidase family
數據庫鏈接：

KEGG: mmu:14067

STRING: 10090.ENSMUSP00000083204

UniGene: Mm.12900

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.