Mouse anti-Smith antibody ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠抗Smith抗體(Sm)酶聯免疫試劑盒（CSB-E15976m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Sm Ab含量。Sm Ab即抗史密斯抗體，是系統性紅斑狼瘡（SLE）的血清標志性抗體。其背景在于SLE是一種自身免疫病。研究機制方面，它能識別細胞核中的特定蛋白，可作為SLE診斷的重要依據，在自身免疫反應和疾病發生發展機制研究中有重要意義。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研領域中對系統性紅斑狼瘡（SLE）相關模型的抗體水平分析；可廣泛應用于小鼠疾病模型中自身抗體動態監測、藥物療效評估或免疫病理學研究；為探索SLE發病機理及潛在治療靶點提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Sm Ab
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-smith antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	92-101
1:8	Average %	93
	Range %	82-98

回收率：

The recovery of mouse anti-smith antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.