Mouse angiotensinogen (aGT) ELISA Kit

文獻引用 (6) 說明書下載

中文名稱：

小鼠血管緊張素原(aGT)酶聯免疫試劑盒
貨號：

CSB-E08566m
規格：

96T/48T
價格：

￥3200/￥2500
其他：

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產品描述：

小鼠血管緊張素原(aGT)酶聯免疫試劑盒（CSB-E08566m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的AGT含量。AGT即血管緊張素原，是腎素-血管緊張素系統的唯一底物。其在血壓調節等生理過程中至關重要。研究機制上，腎素將AGT裂解為血管緊張素I，再經血管緊張素轉換酶生成血管緊張素II，后續作用于相應受體引發系列生理效應。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，其高特異性和靈敏度使其適用于評估疾病模型小鼠的RAS系統激活狀態、藥物干預后aGT表達水平變化，或體外細胞實驗中血管緊張素原分泌動態研究，為心血管、內分泌及腎臟相關基礎科研提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Agt ELISA kit; Serpina8Angiotensinogen ELISA kit; Serpin A8) [Cleaved into: Angiotensin-1 ELISA kit; Angiotensin 1-10 ELISA kit; Angiotensin I ELISA kit; Ang I); Angiotensin-2 ELISA kit; Angiotensin 1-8 ELISA kit; Angiotensin II ELISA kit; Ang II); Angiotensin-3 ELISA kit; Angiotensin 2-8 ELISA kit; Angiotensin III ELISA kit; Ang III ELISA kit; Des-Asp[1]-angiotensin II); Angiotensin-4 ELISA kit; Angiotensin 3-8 ELISA kit; Angiotensin IV ELISA kit; Ang IV); Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4] ELISA kit
縮寫：

AGT
Uniprot No.：

P11859
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse aGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	92-101
1:200	Average %	102
	Range %	98-110
1:400	Average %	91
	Range %	86-95
1:800	Average %	93
	Range %	89-97

回收率：

The recovery of mouse aGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.584	2.445	2.515	2.410
100	1.692	1.573	1.633	1.528
50	0.951	1.016	0.984	0.879
25	0.647	0.662	0.655	0.550
12.5	0.359	0.339	0.349	0.244
6.25	0.236	0.221	0.229	0.124
3.12	0.183	0.187	0.185	0.080
0	0.102	0.108	0.105

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

The protective effect of resveratrol on vascular aging by modulation of the renin–angiotensin system Kim EN,Atherosclerosis,2023
Enhanced Cardiorenal Protective Effects of Combining SGLT2 Inhibition, Endothelin Receptor Antagonism and RAS Blockade in Type 2 Diabetic Mice A Vergara,International journal of molecular sciences,2022
ACE2-like enzyme B38-CAP suppresses abdominal sepsis and severe acute lung injury T Minato,PloS one,2022
Propionate alleviates myocardial ischemia-reperfusion injury aggravated by Angiotensin II dependent on caveolin-1/ACE2 axis through GPR41 Fan Deng,International journal of biological sciences,2022
Propionate alleviates myocardial ischemia-reperfusion injury aggravated by Angiotensin II dependent on caveolin-1/ACE2 axis through GPR41 F Deng,International Journal of Biological Sciences,2021
Opposing effects of reduced kidney mass on liver and skeletal muscle insulin sensitivity in obese mice. Chin SH.et al,Diabetes.,2015

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功能：

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.; acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.; stimulates aldosterone release.; is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
亞細胞定位：

Secreted.
蛋白家族：

Serpin family
組織特異性：

Expressed by the liver and secreted in plasma.
數據庫鏈接：

STRING: 10090.ENSMUSP00000066488

UniGene: Mm.301626

Mouse angiotensinogen (aGT) ELISA Kit

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