Mouse Substance P,SP ELISA Kit

產品詳情

文獻引用 (2) 評價 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠P物質(SP)酶聯免疫試劑盒（CSB-E08359m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的SP含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，廣泛用于基礎科研領域，如探究神經系統疾病模型中SP的分泌動態、評估藥物對神經肽表達的調控作用，或解析炎癥反應與神經信號通路的關聯機制，為神經生物學、免疫學及藥理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

SP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-104
1:200	Average %	94
	Range %	89-98
1:400	Average %	89
	Range %	86-96
1:800	Average %	94
	Range %	89-97

回收率：

The recovery of mouse SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	86	82-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:200

產品評價：我用CSB-E08359m檢測正常血清，其od值為0.7/0.604/0.559/0.639/0.577/0.671

By 張老師