Mouse Spondin-1(SPON1) ELISA kit

說明書下載

產品詳情

產品描述：

小鼠脊椎蛋白-1(SPON1)酶聯免疫試劑盒（CSB-EL022599MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SPON1含量。小鼠脊椎蛋白-1（SPON1）是一種在脊椎動物中廣泛表達的蛋白質，參與細胞外基質構建和細胞粘附。其研究涉及細胞信號傳導、組織修復和腫瘤發生等機制，對理解脊椎發育和疾病治療具有重要意義。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于實驗室環境下的小鼠模型研究，如探究骨骼發育、神經系統疾病或腫瘤微環境中SPON1的調控機制，亦可應用于基因編輯動物或藥物干預實驗中生物標志物的動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Spon1 ELISA Kit; Spondin-1 ELISA Kit; F-spondin ELISA Kit
縮寫：

SPON1
Uniprot No.：

Q8VCC9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	97-106
1:2	Average %	104
	Range %	99-108
1:4	Average %	86
	Range %	81-92
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of mouse SPON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-106
EDTA plasma (n=4)	87	83-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS.
基因功能參考文獻：
1. We conclude that upon ligand binding, TLR4 activates PI3K/Akt signaling to induce F-spondin expression, subsequently control CREB-mediated IL-6 production to promote VSMC migration. These findings provide vital insights into the essential role of F-spondin in VSMC function and will be valuable for developing new therapeutic strategies against atherosclerosis. PMID: 27091427
2. These findings demonstrated novel roles for F-spondin and IL-1Ra in type B CpG oligodeoxynucleotides-mediated cell proliferation and migration of macrophages. PMID: 26042735
3. Spon1 deletion leads to a local and systemic reduction of TGF-beta levels resulting in increased BMP signaling and increased bone deposition in adult mice. PMID: 24875054
4. F-spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization. PMID: 20839318
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
數據庫鏈接：

KEGG: mmu:233744

STRING: 10090.ENSMUSP00000041157

UniGene: Mm.334160