Mouse Signal peptide, CUB and EGF-like domain-containing protein 1(SCUBE1) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠信號肽、CUB和EGF樣結構域蛋白1(SCUBE1)酶聯免疫試劑盒（CSB-EL020866MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SCUBE1含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于基礎科研領域，如探究SCUBE1在疾病模型中的表達調控機制、評估基因敲除或藥物干預后的蛋白水平變化，以及作為相關信號通路研究的生物標志物檢測工具，滿足體外實驗中對SCUBE1蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Scube1 ELISA Kit; Signal peptide ELISA Kit; CUB and EGF-like domain-containing protein 1 ELISA Kit
縮寫：

SCUBE1
Uniprot No.：

Q6NZL8
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	88-95
1:10	Average %	101
	Range %	96-104
1:20	Average %	92
	Range %	87-95
1:40	Average %	98
	Range %	92-101

回收率：

The recovery of mouse SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	107	99-111

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
基因功能參考文獻：
1. SCUBE1 participates in platelet aggregation by bridging adjacent activated platelets in thrombosis. Blockade of soluble SCUBE1 might represent a novel antithrombotic strategy. PMID: 24833801
2. SCUBE1 may be involved in the regulation of tubular cell proliferation and re-epithelialization during the resolution of kidney injury. PMID: 20042401
3. These results support the dual roles of SCUBE1 on brain morphogenesis and cell-cell adhesions through its distinct domain function. PMID: 18303018
4. During tooth development, Scube1 localized to the dental papilla of both incisor and molar teeth, suggestina a role for Scube1 during early craniofacial development. PMID: 19101956
亞細胞定位：

Secreted. Cell membrane; Peripheral membrane protein.
數據庫鏈接：

KEGG: mmu:64706

UniGene: Mm.40393