Mouse SPARC-like protein 1(SPARCL1) ELISA kit

Mouse SPARC-like protein 1(SPARCL1) ELISA kit

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中文名稱：

小鼠SPARC樣蛋白1(SPARCL1)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL022487MO
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

小鼠SPARC樣蛋白1(SPARCL1)酶聯(lián)免疫試劑盒（CSB-EL022487MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SPARCL1含量。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，適用于探索SPARCL1在疾病模型中的表達(dá)動(dòng)態(tài)、細(xì)胞基質(zhì)交互作用機(jī)制或藥物干預(yù)研究，為心血管疾病、纖維化病變及腫瘤相關(guān)基礎(chǔ)研究提供可靠工具?？蓾M足體外實(shí)驗(yàn)、動(dòng)物模型組織分析等科研場(chǎng)景需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Sparcl1 ELISA Kit; Ecm2 ELISA Kit; Sc1 ELISA Kit; SPARC-like protein 1 ELISA Kit; Extracellular matrix protein 2 ELISA Kit; Matrix glycoprotein Sc1 ELISA Kit
縮寫：

SPARCL1
Uniprot No.：

P70663
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPARCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	95
	Range %	90-101
1:4	Average %	94
	Range %	90-97
1:8	Average %	97
	Range %	95-100

回收率：

The recovery of mouse SPARCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	95	91-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.588	2.542	2.565	2.416
2500	1.788	1.745	1.767	1.618
1250	1.079	1.066	1.073	0.924
625	0.698	0.701	0.700	0.551
312.5	0.443	0.434	0.439	0.290
156	0.298	0.278	0.288	0.139
78	0.222	0.230	0.226	0.077
0	0.150	0.148	0.149

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

基因功能參考文獻(xiàn)：
1. tumor endothelial cells heterogeneity is regulated by SPARCL1, which promotes the cell quiescence and vessel homeostasis contributing to the favorable prognoses associated with Th1-tumor microenvironments colorectal carcinomas. PMID: 27721236
2. Study shows that astrocyte-secreted hevin is a trans-synaptic linker that bridges presynaptic NRX1alpha with postsynaptic NL1B. This way, hevin organizes both pre- and postsynaptic specializations and aligns them across the synapse. PMID: 26771491
3. Hevin is transiently expressed in the IrrPTK-injured corneas and loss of hevin predisposes them to aberrant wound healing. PMID: 24303054
4. SC1 and SPARC may share significant homology but are likely to have distinct but complementary roles in central nervous system development. PMID: 22173850
5. This study presented evidence that combined NL1 and SC1 overexpression triggers excitotoxic neurodegeneration through SC1 signaling at synaptic connections initiated by NL1. PMID: 22542674
6. In gliomas implanted into murine brain, a SLF was associated with SPARC in the neovasculature but not with hevin, the latter prominent in the astrocytes encompassed by infiltrating tumor PMID: 21688302
7. These results identify hevin as a positive and SPARC as a negative regulator of synapse formation. PMID: 21788491
8. Hevin does not appear to be critical to regulating intraocular pressure. Hevin is expressed in trabecular meshwork but, in contrast to SPARC, does not appear to be regulated by TGF-beta2. PMID: 21220554
9. proteolysis of hevin by ADAMTS4 in the mouse cerebellum is important for the normal development of this tissue PMID: 20018883
10. determination of collagen I binding and examination of expression in a variety of tissues PMID: 12538579
11. This hematopoietic stem cell enhancer ia bound by transription factors in stem cells and in hemangioblasts. PMID: 17962413
12. Data show that Sparcl1 in the locus Rgcs1 region is highly expressed in the ganglion cell layer and optic nerve head. PMID: 18671875
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亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

SPARC family
組織特異性：

Highest expression in brain. Moderate levels in heart, adrenal gland, epididymis and lung. Low levels in kidney, eye, liver, spleen, submandibular gland and testis.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:13602

STRING: 10090.ENSMUSP00000031249

UniGene: Mm.29027

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.