Mouse Roundabout homolog 4(ROBO4) ELISA kit

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中文名稱：

小鼠環形交叉軸突導向受體同源物4(ROBO4)酶聯免疫試劑盒
貨號：

CSB-EL020057MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

小鼠環形交叉軸突導向受體同源物4(ROBO4)酶聯免疫試劑盒（CSB-EL020057MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ROBO4含量。ROBO4是一個重要靶點。其在血管生成與穩定性維持等方面具關鍵作用。研究機制上，它能參與調節內皮細胞的行為和功能，影響血管的發育和重塑，在腫瘤血管生成等病理過程的研究中受到關注，或為相關疾病治療提供新方向。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，廣泛用于血管生物學、腫瘤微環境、缺血性疾病及炎癥相關機制的基礎研究，為探索內皮細胞功能調控及病理生理過程提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Robo4 ELISA Kit; Roundabout homolog 4 ELISA Kit
縮寫：

ROBO4
Uniprot No.：

Q8C310
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ROBO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-89
1:2	Average %	98
	Range %	94-103
1:4	Average %	86
	Range %	83-89
1:8	Average %	98
	Range %	95-102

回收率：

The recovery of mouse ROBO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-109
EDTA plasma (n=4)	89	85-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.524	2.631	2.578	2.404
5	1.957	1.904	1.931	1.757
2.5	1.240	1.304	1.272	1.098
1.25	0.753	0.768	0.761	0.587
0.625	0.496	0.511	0.504	0.330
0.312	0.302	0.320	0.311	0.137
0.156	0.220	0.234	0.227	0.053
0	0.176	0.172	0.174

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Robo4┸\mediated pancreatic endothelial integrity decreases inflammation and islet destruction in autoimmune diabetes M Troullinaki,FASEB,2020

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功能：

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function.
基因功能參考文獻：
1. Robo4 inhibits angiogenesis and vessel permeability independently of its cytoplasmic domain. Robo4 deletion enhances permeability and revascularization in oxygen-induced retinopathy (OIR) and accelerates cutaneous wound healing. PMID: 27882935
2. endothelial Robo4 signaling is important for suppressing breast cancer growth and metastasis, and it can be targeted (enhanced) by administrating a small molecular drug. PMID: 26778715
3. Robo4 vaccines induce antibodies that retard tumor growth. PMID: 25348086
4. Robo4 and Src family kinases are involved in the mechanisms of embryonic vascular defects caused by the Hrt/Hey deficiency. PMID: 25264302
5. These results clearly indicate that Robo4 plays a role in hematopoietic stem cells trafficking such as bone marrow homing and peripheral mobilization. PMID: 23226403
6. our results showed an important role of Robo4 in the regulation of cortical radial migration through Slit-dependent and -independent mechanisms PMID: 22123939
7. Data found that expression of Slit2 and SDF1 were dynamically regulated in mesenchymal stem cells and OBC-like populations following radiation, while Robo4 expression was restricted to endothelial cells. PMID: 22313734
8. augmenting the host Robo4/Slit2 system could provide a useful therapeutic approach to control pathological angiogenesis associated with HSV induced stromal keratitis PMID: 21572022
9. Robo4 specifically binds to UNC5B, a vascular Netrin receptor, revealing unexpected interactions between two endothelial guidance receptors. PMID: 21238923
10. Activating with the soluble ligand Slit an endothelium-specific, Robo4-dependent signaling pathway that strengthens the vascular barrier, diminishing deleterious aspects of the host's response to the pathogen-induced cytokine storm. PMID: 20375003
11. enhanced growth of blood vessels in Robo4(-/-) endothelium is due to activation of vascular endothelial growth factor (VEGF)-R2 signaling through the Src and FAK kinases. PMID: 20498081
12. Robo4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. PMID: 12941633
13. Robo4 is expressed in hematopoietic stem cells (HSC), and Robo4/Slit2 signaling may play a role in HSC homeostasis in the bone marrow niche. PMID: 18927479
14. Robo4 mRNA level decreases during vascular decline in pulmonary fibrosis. PMID: 17496152
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蛋白家族：

Immunoglobulin superfamily, ROBO family
組織特異性：

Expressed specifically in embryo and adult vascular endothelium.
數據庫鏈接：

KEGG: mmu:74144

STRING: 10090.ENSMUSP00000099959

UniGene: Mm.485070

Mouse Roundabout homolog 4(ROBO4) ELISA kit

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