Mouse Regenerating islet-derived protein 3-alpha(REG3A) ELISA kit

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中文名稱：

小鼠再生胰島衍生蛋白3α(REG3A）酶聯免疫試劑盒
貨號：

CSB-EL019548MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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小鼠再生胰島衍生蛋白3α(REG3A）酶聯免疫試劑盒（CSB-EL019548MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的REG3A含量。REG3A作為一個靶點在研究中備受關注。它在機體的多種生理和病理過程中發揮作用。研究機制上，主要聚焦于其在免疫調節、組織修復及腫瘤發生發展等方面的功能，揭示其信號通路及與其他分子的相互作用，為相關疾病治療提供思路。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于研究REG3A在糖尿病動物模型中的動態變化、腸道炎癥機制探索或組織修復過程中的調控作用，為疾病機制研究、藥物療效評估及分子通路分析提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Reg3a ELISA Kit; Pap2 ELISA Kit; Regenerating islet-derived protein 3-alpha ELISA Kit; REG-3-alpha ELISA Kit; Islet of Langerhans regenerating protein 3 ELISA Kit; Lithostathine 3 ELISA Kit; Pancreatitis-associated protein 2 ELISA Kit; Regenerating islet-derived protein III-alpha ELISA Kit; Reg III-alpha) [Cleaved into: Regenerating islet-derived protein 3-alpha 16.5 kDa form; Regenerating islet-derived protein 3-alpha 15 kDa form] ELISA Kit
縮寫：

REG3A
Uniprot No.：

O09037
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-106
1:2	Average %	98
	Range %	96-104
1:4	Average %	88
	Range %	86-95
1:8	Average %	96
	Range %	91-102

回收率：

The recovery of mouse REG3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	88	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.360	2.512	2.436	2.311
100	1.831	1.987	1.909	1.784
50	1.252	1.254	1.253	1.128
25	0.747	0.766	0.757	0.632
12.5	0.473	0.512	0.493	0.368
6.25	0.315	0.321	0.318	0.193
3.12	0.219	0.217	0.218	0.093
0	0.124	0.126	0.125

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Decreased IL-17RB expression impairs CD11b+CD11c-myeloid cell accumulation in gastric mucosa and host defense during the early-phase of Helicobacter pylori infection Teng YS, et al,Cell Death & Disease,2019

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功能：

Bactericidal C-type lectin. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
基因功能參考文獻：
1. skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing PMID: 28774595
2. Reg3alpha is an extracellular matrix-targeted reactive oxygen species scavenger that binds the fibrin scaffold resulting from hepatocyte death during acute liver failure. PMID: 25938566
3. Data show that regenerating islet-derived 3-alpha (REG3alpha) is a plasma biomarker of gastrointestinal tract graft-versus-host disease that can be combined with clinical stage and histologic grade to improve risk stratification of patients. PMID: 21979939
4. Reg3alpha stimulates beta-cell replication, by activating Akt kinase and increasing the levels of cyclin D1/CDK4. PMID: 19343564
相關疾病：

Overexpressed during the acute phase of pancreatitis.
亞細胞定位：

Secreted.
組織特異性：

Small intestine and pancreas.
數據庫鏈接：

KEGG: mmu:19694

STRING: 10090.ENSMUSP00000098829

UniGene: Mm.2552

Mouse Regenerating islet-derived protein 3-alpha(REG3A) ELISA kit

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