Mouse Proto-oncogene Wnt-3(WNT3) ELISA kit
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中文名稱：小鼠原癌基因Wnt-3蛋白(WNT3)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL026135MO
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠原癌基因Wnt-3蛋白(WNT3)酶聯(lián)免疫試劑盒（CSB-EL026135MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的WNT3含量。WNT3作為一種靶點(diǎn)，屬于Wnt蛋白家族。它在胚胎發(fā)育、細(xì)胞增殖和分化等過程中發(fā)揮重要作用。其異常表達(dá)與腫瘤發(fā)生、發(fā)展密切相關(guān)。研究主要聚焦于其信號(hào)通路機(jī)制，旨在通過干預(yù)該靶點(diǎn)調(diào)控相關(guān)信號(hào)，為疾病治療提供新策略。試劑盒檢測(cè)范圍為39.07 pg/mL-2500 pg/mL，該產(chǎn)品可廣泛應(yīng)用于基礎(chǔ)研究領(lǐng)域，包括Wnt信號(hào)通路調(diào)控機(jī)制解析、基因功能驗(yàn)證、藥物靶點(diǎn)篩選及疾病動(dòng)物模型構(gòu)建等科研場(chǎng)景，為探索WNT3在細(xì)胞增殖分化、腫瘤微環(huán)境調(diào)控及再生醫(yī)學(xué)研究中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Wnt3 ELISA Kit; Int-4 ELISA Kit; Wnt-3 ELISA Kit; Proto-oncogene Wnt-3 ELISA Kit; Proto-oncogene Int-4 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測(cè)范圍：39 pg/ml-2500 pg/ml
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靈敏度：9.75 pg/ml
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Stem Cells
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 86 Range % 82-91 1:2 Average % 101 Range % 98-105 1:4 Average % 87 Range % 84-91 1:8 Average % 100 Range % 95-105 -
回收率：
The recovery of mouse WNT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-94 EDTA plasma (n=4) 107 103-110 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2500 2.547 2.601 2.574 2.411 1250 2.291 2.200 2.246 2.083 625 1.543 1.601 1.572 1.409 312.5 0.971 0.968 0.970 0.807 156.25 0.572 0.601 0.587 0.424 78.13 0.414 0.400 0.407 0.244 39.07 0.295 0.290 0.293 0.130 0 0.161 0.164 0.163 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Does murine spermatogenesis require WNT signalling? A lesson from Gpr177 conditional knockout mouse models. Chen SR.et al,Cell Death Dis.,2016
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge and for normal embryonic limb development.
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基因功能參考文獻(xiàn)：
- Downregulation of Gata4 could restore the phenotype exhibited by Wnt3 downregulation in dorsal root ganglion neurons. PMID: 29567480
- Here, we describe the culture protocol using Wnt-3a for successful maintenance of skin epithelial stem cells . PMID: 27032938
- stem-cell membranes constitute a reservoir for Wnt proteins, while Frizzled receptor turnover and 'plasma membrane dilution' through cell division shape the epithelial Wnt3 gradient PMID: 26863187
- timed secretion of wnt3 by ingrowing axons from the thalamus regulates the combinatorial composition of ribosomal proteins in developing neocortex. WNT3 further regulates the specificity of mRNA translation and development of neurons and oligodendrocytes. PMID: 26245956
- this article shows that absence of Wnt3 in the posterior visceral endoderm leads to delayed formation of the primitive streak and that interplay between anterior and posterior visceral endoderm restricts the position of the primitive streak PMID: 25907228
- Data indicate that functional Wnt3/beta-Catenin signaling is localized to the dorsal subventricular zone (SVZ) microdomain. PMID: 24449255
- Wnt3 is part of a signaling loop that affects homeostasis of intestinal stem and Paneth cells in mice. PMID: 22922422
- Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice PMID: 23085236
- These results suggest that the regulation of paracrine factors plays a critical role in hippocampal aging and neurogenesis. PMID: 21746862
- Wnt-3a and Wnt-3 factors signal through the canonical Wnt/beta-catenin pathway to regulate different aspects of spinal cord neural progenitor development. PMID: 20722074
- The mRNA levels of Wnt3, 6, and 9a were significantly upregulated in the intestinal epithelial cells by Salmonella. PMID: 20083111
- Different permissive Wnt3/beta-catenin signaling thresholds is required for the embryonic development of head structures and adult intestinal polyposis. PMID: 20084116
- WNT-3, expressed by motor neurons, acts as a retrograde signal that controls terminal arborization of muscle afferents. PMID: 12354395
- Expression is regulated by beta-catenin during formation of the anteroposterior axis and the mesoderm PMID: 14623818
- effects of Wnt3a are exerted through activation of the beta-catenin signal transduction pathway and do not require alternative Wnt transduction cascades PMID: 15009701
- Data suggest that recruitment of Axin and Frat1 to the membrane by LRP5-triggered Wnt3 signaling leads to both Axin degradation and Frat1-mediated inhibition of glycogen synthase kinase-3. PMID: 15699046
- Wnt3a and SB216763 promoted the proliferation of retinal stem cells, partly dependent on FGF2 signaling.A combination of Wnt and FGF signaling may provide a therapeutic strategy for in vitro expansion or in vivo activation of adult retinal stem cells. PMID: 16223856
- Wnt3 repulsion is mediated by Ryk, expressed in a ventral-to-dorsal decreasing gradient PMID: 16280981
- We further delineate the role of Wnt signaling pathway as evidenced by induction of Wnt3 and Wnt8b, stimulation of FRP-1, inhibition of GSK-B, accumulation of cytosolic beta-catenin, and transcription of target genes, including c-myc and cyclin-D1. PMID: 16522160
- This study findings reveal the rich contributions of the Wnt3a lineage to a variety of brain structures and show that functionally integrated nuclei can share a molecular identity. PMID: 17701978
- Wnt3 expression in the epiblast is required for induction of the primitive streak and mesoderm whereas activity in the posterior visceral endoderm is dispensable. PMID: 18028899
- an internally truncated LRP5 receptor is strongly implicated in deregulated activation of the WNT/beta-catenin signaling pathway in hyperparathyroid tumors PMID: 18044981
- head development is sensitive to the level of WNT3 signalling and that DKK1 is the key antagonist that modulates WNT3 activity during anterior morphogenesis. PMID: 18403408
- Wnt-3a signaling regulates hemopoiesis through stromal cells with selectivity and different degrees of the effect, depending on the hemopoietic lineage in the hemopoietic microenvironment. PMID: 11441081
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亞細(xì)胞定位：Secreted, extracellular space, extracellular matrix. Secreted.
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蛋白家族：Wnt family
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組織特異性：Detected at low levels in adult brain. Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds.
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數(shù)據(jù)庫鏈接：
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