Mouse Protein notum homolog(NOTUM) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠notum蛋白同源物(NOTUM)酶聯免疫試劑盒（CSB-EL015955MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養上清液樣本中的NOTUM含量。NOTUM是一個重要靶點。它是分泌性蛋白，可調節Wnt信號通路。異常激活的Wnt信號與多種疾病相關，如腫瘤、纖維化等。對NOTUM的研究，主要圍繞其對Wnt信號的調控機制，期望通過干預該靶點為相關疾病治療提供新思路。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于體外研究NOTUM在胚胎發育、干細胞分化以及骨代謝相關疾病模型中的表達動態，尤其適合評估不同處理條件下（如基因編輯、藥物干預等）細胞或組織內源性NOTUM的分泌水平變化，為探索Wnt信號通路調控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

NotumPalmitoleoyl-protein carboxylesterase NOTUM ELISA Kit; EC 3.1.1.98 ELISA Kit
縮寫：

NOTUM
Uniprot No.：

Q8R116
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	109
	Range %	104-113
1:2	Average %	93
	Range %	89-99
1:4	Average %	100
	Range %	96-104
1:8	Average %	88
	Range %	84-92

回收率：

The recovery of mouse NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	98	94-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
基因功能參考文獻：
1. Notum influences mesenchymal cell differentiation by generating a barrier for Wnt ligands produced and secreted by airway epithelial cells to attenuate Wnt signaling. PMID: 29428562
2. The findings support a significant role for NOTUM in modulating WNT signaling pathways that have pleiotropic effects on tooth and kidney development. PMID: 26926082
3. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
4. Male mice deficient in Notum specifically in the liver develop metabolic dysfunctions implicating Notum in the development of Type 2 diabetes. PMID: 26974334
5. Describes the human ortholog, and analyzes both the human and mouse promoters. PMID: 18429952
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亞細胞定位：

Secreted.
蛋白家族：

Pectinacetylesterase family, Notum subfamily
組織特異性：

Widely expressed. Expressed in lung, ovary, kidney, liver and brain. Not detected in thymus, heart, spleen, stomach, skeletal muscle and bone marrow.
數據庫鏈接：

KEGG: mmu:77583

STRING: 10090.ENSMUSP00000101783

UniGene: Mm.32839