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中文名稱:小鼠降鈣素原(PCT)酶聯免疫試劑盒
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貨號:CSB-E10371m
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規格:96T/48T
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價格:¥3800/¥2500
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其他:
產品詳情
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產品描述:小鼠降鈣素原(PCT)酶聯免疫試劑盒(CSB-E10371m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的PCT含量。降鈣素原(PCT)是一種蛋白質。在健康人體中,PCT 水平極低。當發生嚴重細菌、真菌、寄生蟲感染及膿毒癥和多臟器功能衰竭時,其水平會顯著升高。研究認為,它可作為炎癥和感染的生物標志物,輔助醫生判斷病情和指導治療。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,可應用于科研場景中PCT相關機制研究。科研人員可將其應用于實驗動物模型中膿毒癥、全身性感染及炎癥性疾病的機制探索,或用于評估藥物干預對炎癥通路的影響。本試劑盒支持多種生物樣本類型檢測,為實驗室開展PCT動態監測、病原體 - 宿主互作研究及免疫調節實驗提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:N/A
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縮寫:PCT
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:1.56 pg/mL-100 pg/mL
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靈敏度:0.39 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 91 Range % 88-93 1:40 Average % 102 Range % 99-104 1:80 Average % 94 Range % 89-96 1:160 Average % 97 Range % 94-101 -
回收率:
The recovery of mouse PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-97 EDTA plasma (n=4) 96 90-98 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 100 2.224 2.304 2.264 2.090 50 1.655 1.685 1.670 1.496 25 1.122 1.157 1.140 0.966 12.5 0.732 0.721 0.727 0.553 6.25 0.441 0.424 0.433 0.259 3.12 0.315 0.302 0.309 0.135 1.56 0.242 0.228 0.235 0.061 0 0.176 0.172 0.174 -
數據處理:
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貨期:3-5 working days
引用文獻
- ACE2 shedding exacerbates sepsis-induced gut leak via loss of microbial metabolite 5-methoxytryptophan J Gong, H Lu, Y Li, Q Xu, Y Ma, A Lou, W Cui, W Song,Microbiome,2025
- Transcript-dependent effects of the CALCA gene on the progression of post-traumatic osteoarthritis in mice S Jiang,Communications biology,2024
- Honokiol Prevents Intestinal Barrier Dysfunction in Mice with Severe Acute Pancreatitis and Inhibits the JAK/STAT1 Pathway and Acetylation of HMGB1 J Li,/,2023
- Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment Anke Baranowsky,Bone Research,2022
- Safety and Therapeutic Efficacy of Thymoquinone-Loaded Liposomes against Drug-Sensitive and Drug-Resistant Acinetobacter baumannii KS Allemailem,Pharmaceutics,2021
- Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes M Wu,Journal of Advanced Research,2020
- Effect of small molecular weight soybean protein-derived peptide supplementation on attenuating burn injury-induced inflammation and accelerating wound healing in a rat model Fen Zhao, et al,Rsc Advances,2019
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