Mouse Osteocrin(OSTN) ELISA kit

Mouse Osteocrin(OSTN) ELISA kit

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中文名稱：

小鼠骨織素(OSTN)酶聯免疫試劑盒
貨號：

CSB-EL017269MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

小鼠骨織素(OSTN)酶聯免疫試劑盒（CSB-EL017269MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的OSTN含量。OSTN即骨鈣素，是一種分泌型骨細胞因子。它在骨骼發育、礦化等過程中發揮重要作用。研究機制上，其能調節成骨細胞與破骨細胞的活動，影響骨代謝平衡，還可參與能量代謝、糖脂代謝等，有望成為骨代謝疾病等領域的治療靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于能量代謝研究、骨發育調控機制探索、運動生理學實驗等科研領域。可廣泛應用于肥胖模型、骨質疏松癥研究或生物節律相關實驗中的OSTN動態監測，為體外培養細胞或動物模型中的OSTN表達分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ostn ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
縮寫：

OSTN
Uniprot No.：

P61364
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Stem Cells
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	87-97
1:10	Average %	95
	Range %	88-99
1:20	Average %	93
	Range %	87-100
1:40	Average %	95
	Range %	87-104

回收率：

The recovery of mouse OSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-99
EDTA plasma (n=4)	97	89-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.579	2.605	2.592	2.502
500	1.447	1.494	1.471	1.381
250	0.791	0.803	0.797	0.707
125	0.430	0.427	0.429	0.339
62.5	0.219	0.225	0.222	0.132
31.2	0.144	0.149	0.147	0.057
15.6	0.107	0.112	0.110	0.020
0	0.088	0.091	0.090

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

MyoRep: a novel reporter system to detect early muscle atrophy in vitro and in vivo AD Re Cecconi, N Rizzi, M Barone, F Palo, M Lunardi,bioRxiv,2025
Exercise-induced Musclin determines the fate of fibro-adipogenic progenitors to control muscle homeostasis J Lyu,Cell stem cell,2024

功能：

Hormone that acts as a ligand for natriuretic peptide receptor NPR3/NPR-C and promotes bone growth and physical endurance in muscle. Acts as a regulator of osteoblast differentiation and bone growth by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production. Required to enhance physical endurance: induced following physical exercise in muscle and promotes cGMP production, probably by interacting with NPR3/NPR-C. May act as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle.
基因功能參考文獻：
1. The mechanism underlying the effect of OSTN on skeletal growth relevant to C-type natriuretic peptide /Natriuretic peptide receptor B signaling. PMID: 28990933
2. Data show that musclin (Ostn) production is stimulated by Ca2+-dependent proto-oncogene protein c-Akt (Akt) phosphorylation. PMID: 26668395
3. findings suggest that palmitate increases musclin gene expression via the activation of the PERK signaling pathway in C2C12 myotubes PMID: 26449458
4. osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID: 14523025
5. Musclin expression level is tightly regulated by nutritional changes and its physiological role could be linked to glucose metabolism PMID: 15044443
6. Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID: 17950246
7. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249
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亞細胞定位：

Secreted.
蛋白家族：

Osteocrin family
組織特異性：

Expressed in skeletal muscle and to a much lesser extent in bone, brown adipose tissue, spleen and testis. Not expressed in neurons.
數據庫鏈接：

KEGG: mmu:239790

UniGene: Mm.390880

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.