Mouse Neutrophil defensin 4(DEFA4) ELISA kit

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中文名稱：

小鼠中性粒細胞防御素4(DEFA4)酶聯(lián)免疫試劑盒
貨號：

CSB-EL006656MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠中性粒細胞防御素4(DEFA4)酶聯(lián)免疫試劑盒（CSB-EL006656MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的DEFA4含量。DEFA4是一種靶點。它在生物體內(nèi)可能具有特定生理功能。目前對于它的研究，主要聚焦于其在相關(guān)生理和病理過程中的作用機制，探索它與疾病發(fā)生發(fā)展的聯(lián)系，為開發(fā)針對該靶點的治療手段、藥物等提供理論依據(jù)。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于科研領(lǐng)域中小鼠模型感染性疾病機制研究、炎癥反應(yīng)評估或免疫調(diào)控實驗，例如通過檢測不同病理狀態(tài)下DEFA4的表達水平變化，探索其在宿主防御系統(tǒng)中的作用，亦可用于藥物開發(fā)中免疫調(diào)節(jié)劑的效果驗證。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Defa4 ELISA kit; Defcr4Alpha-defensin 4 ELISA kit; Defensin-related cryptdin-4 ELISA kit
縮寫：

DEFA4
Uniprot No.：

P28311
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse DEFA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	93
	Range %	90-96
1:4	Average %	88
	Range %	84-92
1:8	Average %	101
	Range %	97-105

回收率：

The recovery of mouse DEFA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	83	80-86

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.654	2.689	2.672	2.515
10	2.059	2.001	2.030	1.873
5	1.312	1.335	1.324	1.167
2.5	0.852	0.868	0.860	0.703
1.25	0.505	0.512	0.509	0.352
0.625	0.373	0.360	0.367	0.210
0.312	0.266	0.268	0.267	0.110
0	0.158	0.156	0.157

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Glucagon-like peptide-2 improves intestinal immune function and diminishes bacterial translocation in a mouse model of parenteral nutrition Lei Q,Nutrition research,2023

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功能：

Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
基因功能參考文獻：
1. Depleting CRP4 is incompatible with a proper cardiac development and function. CRP4 is a novel antiremodeling factor regulated, at least to some extent, by cardiac cGMP. PMID: 28138039
2. Backbone cyclization provides a means for engineeringalpha-defensins to improve their biological activity while providing excellent stability to serum degradation and highlighting the potential of peptide scaffolds for development of novel compounds. PMID: 22040603
3. bactericidal activity predominantly affects noncommensal bacteria PMID: 21099205
4. the prosegment of Crp4 inhibits Crp4 bactericidal activity by blocking peptide-mediated perturbation of target cell membranes PMID: 12574157
5. Crp4 disulfide arrangement confers essential protection from degradation by this critical activating proteinase, allowing antibacterial action. PMID: 15297466
6. function of the conserved salt bridge in Crp4 is not linked to bactericidal activity or proteolytic stability of the mature peptide. PMID: 16857681
7. CRP4 mediates cGMP/PKG stimulation of SM-specific gene expression, and PKG plays an important role in regulating the phenotype of VSMCs PMID: 17878170
8. Kinetics of Crp4 translocation coupled with peptide-induced vesicle leakage are reported. PMID: 17910476
9. Beta-sheet-containing Crp4 induces membrane permeabilization more effectively than disulfide-null Crp4 by translocating and forming transient membrane defects. PMID: 18973303
10. Lys-for-Arg replacements in cryptdin 4 attenuated bactericidal activity PMID: 19737896
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亞細胞定位：

Secreted.
蛋白家族：

Alpha-defensin family
組織特異性：

Paneth cells of the small bowel.
數(shù)據(jù)庫鏈接：

KEGG: mmu:13238

UniGene: Mm.426634

Mouse Neutrophil defensin 4(DEFA4) ELISA kit

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