Mouse Leukotriene B4,LT-B4 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠白三烯B4(LTB4)酶聯(lián)免疫試劑盒（CSB-E08034m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的LT-B4含量。LT-B4，即白三烯B4，是一種由花生四烯酸代謝生成的生物活性物質(zhì)，具有高度炎癥反應(yīng)性。它在急性髓系白血病（AML）患者中表達(dá)水平異常，與不良預(yù)后相關(guān)，可能通過調(diào)控免疫微環(huán)境、促進(jìn)腫瘤增殖和侵襲等機(jī)制影響AML的發(fā)生發(fā)展。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于體外實(shí)驗(yàn)中對(duì)炎癥機(jī)制、免疫調(diào)節(jié)或藥物干預(yù)效果的評(píng)估研究；為炎癥性疾病模型研究、免疫調(diào)控機(jī)制探索等科研項(xiàng)目提供可靠的實(shí)驗(yàn)數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

LT-B4
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	91
	Range %	86-97
1:4	Average %	95
	Range %	91-99
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of mouse LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-101
EDTA plasma (n=4)	93	89-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.