Mouse Immunoglobulin A, IgA ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠免疫球蛋白A(IgA)酶聯免疫試劑盒（CSB-E07986m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgA含量。IgA是一種免疫球蛋白，主要存在于黏膜表面，具有抗菌和抗病毒作用。IgA腎病是一種以IgA沉積在腎小球系膜區為特征的腎小球疾病，其發病機制復雜，涉及遺傳、環境因素和免疫反應等多重打擊假說。目前，針對IgA腎病的研究主要集中在靶向B細胞免疫通路、補體系統等靶點，以開發更有效的治療藥物。試劑盒檢測范圍為25 ng/mL-1600 ng/mL，腸道菌群研究、呼吸道感染模型評估、免疫調節劑藥效分析、小鼠感染性疾病模型、自身免疫疾病研究、過敏反應實驗中黏膜免疫水平的動態監測、免疫機制探索、生物標志物分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgA
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

25 ng/mL-1600 ng/mL
靈敏度：

25 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	93
	Range %	88-95
1:10000	Average %	95
	Range %	90-99
1:20000	Average %	89
	Range %	85-94
1:40000	Average %	97
	Range %	91-102

回收率：

The recovery of mouse IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	102	98-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.