Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠羥基酸氧化酶1(HAO1)酶聯免疫試劑盒（CSB-EL010127MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的HAO1含量。小鼠羥基酸氧化酶1（HAO1）是草酸代謝中的關鍵酶，其表達下調可顯著降低細胞草酸生成，具有作為干預細胞草酸生成靶點的潛力。研究機制涉及HAO1在氧化應激損傷中的作用，以及其對相關疾病如糖尿病和腫瘤轉移的影響。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于探索HAO1在代謝通路中的分子機制、評估基因敲除/過表達模型小鼠的酶活性變化，或研究藥物干預、營養狀態等因素對HAO1表達的影響，為脂質代謝相關基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Hao1 ELISA Kit; Gox1 ELISA Kit; Hao-1 ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; HAOX1 ELISA Kit; EC 1.1.3.15 ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit
縮寫：

HAO1
Uniprot No.：

Q9WU19
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	83-107
1:2	Average %	95
	Range %	89-103
1:4	Average %	92
	Range %	85-105
1:8	Average %	91
	Range %	87-99

回收率：

The recovery of mouse HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-103
EDTA plasma (n=4)	96	90-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
亞細胞定位：

Peroxisome.
蛋白家族：

FMN-dependent alpha-hydroxy acid dehydrogenase family
組織特異性：

Liver.
數據庫鏈接：

KEGG: mmu:15112

STRING: 10090.ENSMUSP00000028704

UniGene: Mm.26634