Mouse Histone deacetylase complex subunit SAP130(SAP130) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠組蛋白去乙酰化酶復合物亞基SAP130(SAP130)酶聯免疫試劑盒（CSB-EL020694MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的SAP130含量。試劑盒檢測范圍為46.88 pg/mL-3000 pg/mL，本產品適用于基礎科研領域，如探究表觀遺傳調控機制、建立炎癥相關疾病動物模型、評估藥物干預對SAP130表達的影響等研究場景，為探索SAP130在免疫調控和疾病發生中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Sap130 ELISA Kit; Histone deacetylase complex subunit SAP130 ELISA Kit; 130 kDa Sin3-associated polypeptide ELISA Kit; Sin3-associated polypeptide p130 ELISA Kit
縮寫：

SAP130
Uniprot No.：

Q8BIH0
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

46.88 pg/mL-3000 pg/mL
靈敏度：

11.72 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAP130 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	98
	Range %	93-102
1:4	Average %	93
	Range %	89-98
1:8	Average %	91
	Range %	87-97

回收率：

The recovery of mouse SAP130 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	94	93-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
亞細胞定位：

Nucleus.
蛋白家族：

SAP130 family
數據庫鏈接：

KEGG: mmu:269003

STRING: 10090.ENSMUSP00000025109

UniGene: Mm.194538