Mouse Glucose-6-phosphate isomerase(GPI) ELISA kit

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中文名稱：

小鼠果糖-6-磷酸異構(gòu)酶(GPI)酶聯(lián)免疫試劑盒
貨號：

CSB-EL009717MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

小鼠果糖-6-磷酸異構(gòu)酶(GPI)酶聯(lián)免疫試劑盒（CSB-EL009717MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GPI含量。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于基礎科研中能量代謝相關模型構(gòu)建、藥物干預效應評估及分子機制探索等研究場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Gpi ELISA Kit; Gpi1Glucose-6-phosphate isomerase ELISA Kit; GPI ELISA Kit; EC 5.3.1.9 ELISA Kit; Autocrine motility factor ELISA Kit; AMF ELISA Kit; Neuroleukin ELISA Kit; NLK ELISA Kit; Phosphoglucose isomerase ELISA Kit; PGI ELISA Kit; Phosphohexose isomerase ELISA Kit; PHI ELISA Kit
縮寫：

GPI
Uniprot No.：

P06745
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	94
	Range %	87-98
1:4	Average %	100
	Range %	94-105
1:8	Average %	97
	Range %	91-102

回收率：

The recovery of mouse GPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-102
EDTA plasma (n=4)	102	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.538	2.422	2.480	2.308
25	1.736	1.684	1.710	1.538
12.5	1.197	1.143	1.170	0.998
6.25	0.689	0.681	0.685	0.513
3.12	0.414	0.389	0.402	0.230
1.56	0.295	0.285	0.290	0.118
0.78	0.223	0.226	0.225	0.053
0	0.174	0.170	0.172

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis S Liu,Immunity,2021

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功能：

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
基因功能參考文獻：
1. PGI is a moonlighting protein that functions as a cytosolic enzyme involved in glycolysis and gluconeogenesis, and as cytokine through binding to its cell surface receptor. PMID: 8674049
2. The mGPI-transgenic mice presented GPI more efficiently and showed a dramatic increase in negative selection and an inhibition of arthritis. PMID: 23840022
3. Data show that silencing of AMF/PGI induced MET and suppressed the tumor growth and pulmonary metastasis of LM8 cells in nude mice. PMID: 20978190
4. induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid (tumor autocrine motility factor) PMID: 12054583
5. acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils PMID: 12888574
6. Gpi1 immunoreactivity was found in ganglion, amacrine, horizontal and bipolar cells, but not in rods, cones, pigment epithelium and Muller cells. PMID: 15182299
7. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. PMID: 15342241
8. crystal structure of Autocrine Motility Factor PMID: 15502335
9. AMF expression significantly contributes to the aggressive phenotype of pancreatic cancer PMID: 15570012
10. Results report the first high-resolution crystal structure of the inhibitor-free form and the eight types of inhibitor complexes of mouse autocrine motility factor. PMID: 16375918
11. the receptor molecule for AMF/NLK/MF in leukemic differentiation is not gp78 PMID: 17071500
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亞細胞定位：

Cytoplasm. Secreted.
蛋白家族：

GPI family
數(shù)據(jù)庫鏈接：

KEGG: mmu:14751

STRING: 10090.ENSMUSP00000049355

UniGene: Mm.589

Mouse Glucose-6-phosphate isomerase(GPI) ELISA kit

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