Mouse Fibrinogen-like protein 1(FGL1) ELISA kit

Mouse Fibrinogen-like protein 1(FGL1) ELISA kit

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中文名稱：

小鼠纖維蛋白原樣蛋白1(FGL1)酶聯(lián)免疫試劑盒
貨號：

CSB-EL008653MO
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

小鼠纖維蛋白原樣蛋白1(FGL1)酶聯(lián)免疫試劑盒（CSB-EL008653MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGL1含量。FGL1是一種在腫瘤細(xì)胞和肝細(xì)胞中表達(dá)的免疫檢查點分子，其表達(dá)在多種腫瘤中上調(diào)，與抗PD-L1/PD-1治療的耐藥性和預(yù)后不良有關(guān)。研究揭示FGL1通過作為LAG-3的主要配體抑制T細(xì)胞活化，其乙酰化修飾和泛素化降解調(diào)控腫瘤免疫逃逸。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于基礎(chǔ)科研中探究肝臟代謝功能、免疫調(diào)節(jié)機(jī)制、腫瘤微環(huán)境等領(lǐng)域的FGL1動態(tài)表達(dá)研究，為動物模型構(gòu)建、藥物干預(yù)效果評估及分子機(jī)制解析提供可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Fgl1 ELISA Kit; Mfire1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Fibrinogen-related protein 1 ELISA Kit; Mfrep-1 ELISA Kit; Liver fibrinogen-related protein-1 ELISA Kit; Mfire-1 ELISA Kit
縮寫：

FGL1
Uniprot No.：

Q71KU9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-99
1:200	Average %	84
	Range %	81-89
1:400	Average %	83
	Range %	90-96
1:800	Average %	87
	Range %	84-90

回收率：

The recovery of mouse FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	107	104-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.076	0.079	0.078
1000	0.135	0.146	0.141
500	0.206	0.220	0.213
250	0.362	0.379	0.371
125	0.593	0.586	0.590
62.5	0.901	0.932	0.917
31.25	1.213	1.236	1.225
0	2.054	2.098	2.076

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation T Tian,Cell reports,2023
Plasma Fibrinogen-Like 1 as a Potential Biomarker for Radiation-Induced Liver Injury Han NK, et al,Cells,2019
A novel hepatokine, HFREP1, plays a crucial role in the development of insulin resistance and type 2 diabetes Wu HT.et al,Diabetologia.,2016

功能：

Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3. Responsible for LAG3 T-cell inhibitory function. Binds LAG3 independently from MHC class II (MHC-II). Secreted by, and promotes growth of, hepatocytes.
基因功能參考文獻(xiàn)：
1. The results suggest that Fgl1 acts as a tumor suppressor in hepatocellular cancer through an Akt-dependent mechanism and support its role as a potential therapeutic target in hepatocellular carcinoma. PMID: 26225745
2. Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
3. Fgl1 is a member of an emerging group of proteins with key roles in metabolism and liver regeneration. PMID: 23483972
4. HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666
亞細(xì)胞定位：

Secreted.
組織特異性：

Mainly expressed in liver. Also expressed in brown adipose tissue.
數(shù)據(jù)庫鏈接：

KEGG: mmu:234199

STRING: 10090.ENSMUSP00000034003

UniGene: Mm.277955

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.