Mouse EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit
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中文名稱：小鼠含EGF纖維蛋白細(xì)胞外基質(zhì)蛋白 1(EFEMP1)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL007450MO
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠含EGF纖維蛋白細(xì)胞外基質(zhì)蛋白 1(EFEMP1)酶聯(lián)免疫試劑盒（CSB-EL007450MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的EFEMP1含量。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品可為研究EFEMP1在小鼠模型中的生物學(xué)功能提供可靠工具，例如探索其在組織修復(fù)、腫瘤轉(zhuǎn)移或眼部疾病中的表達(dá)動(dòng)態(tài)，亦可用于基因編輯動(dòng)物模型的表型驗(yàn)證及藥物干預(yù)實(shí)驗(yàn)的效果評(píng)估，滿足基礎(chǔ)科研中對(duì)EFEMP1蛋白水平的精準(zhǔn)定量需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Efemp1 ELISA Kit; Fbln3EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Fibulin-3 ELISA Kit; FIBL-3 ELISA Kit
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縮寫：EFEMP1
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測(cè)范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.81 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Neuroscience
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 92-100 1:2 Average % 88 Range % 84-92 1:4 Average % 92 Range % 88-96 1:8 Average % 104 Range % 100-108 -
回收率：
The recovery of mouse EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 85-94 EDTA plasma (n=4) 97 93-101 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.243 2.168 2.206 2.033 1000 1.553 1.605 1.579 1.406 500 1.016 1.013 1.015 0.842 250 0.597 0.574 0.586 0.413 125 0.388 0.412 0.400 0.227 62.5 0.324 0.337 0.331 0.158 31.25 0.277 0.271 0.274 0.101 0 0.177 0.169 0.173 -
數(shù)據(jù)處理：
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貨期：3-5 working days
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相關(guān)問答
■ 常見問題解答
Q:
Regarding your ELISA kit CSB-EL007450MO, a customer is about to use it and they harvested their cells (for cell lysate samples) in RIPA buffer rather than PBS. Do you know if RIPA buffer would cause any negative effects with this kit, or is RIPA going to be expected to be OK?
A:
Thanks for your inquiry!
Generally speaking, RIPA will contain SDS, Triton X-100, NP-40 and other components. Customers now need to confirm the RIPA buffer formula. Pls note that it can not contain SDS (it will destroy the hydrogen bonds of the protein, which will lead to protein denaturation.);
For Triton X-100, NP-40, you need to control the low concentration range (generally not more than 1%, but it is not verified); For the entire RIPA buffer, sorry we have not tried this preparation method before, so we can only suggest you do a pretest if you want to try it.
Pls let me know if you have any further questions. Thank you.
Generally speaking, RIPA will contain SDS, Triton X-100, NP-40 and other components. Customers now need to confirm the RIPA buffer formula. Pls note that it can not contain SDS (it will destroy the hydrogen bonds of the protein, which will lead to protein denaturation.);
For Triton X-100, NP-40, you need to control the low concentration range (generally not more than 1%, but it is not verified); For the entire RIPA buffer, sorry we have not tried this preparation method before, so we can only suggest you do a pretest if you want to try it.
Pls let me know if you have any further questions. Thank you.
Q:
Can you please advise on the immunogens used to raise the antibodies from this kit? Were they full-length or partial-length? And were the antibodies polyclonal or monoclonal?
A:
Thanks for your inquiry!CSB-EL007450MO
Capture antibody is rat monoclonal antibody and immunogen is NS0-derived recombinant protein,full-length.
Detection antibody is goat polyclonal antibody and immunogen is
NS0-derived recombinant protein,partial.
Capture antibody is rat monoclonal antibody and immunogen is NS0-derived recombinant protein,full-length.
Detection antibody is goat polyclonal antibody and immunogen is
NS0-derived recombinant protein,partial.
靶點(diǎn)詳情
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功能：Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
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基因功能參考文獻(xiàn)：
- TWEAK promotes migration and invasion in murine embryonic fibroblasts through a mechanism dependent on ERKs activation and Fibulin 3 down-regulation. PMID: 28383766
- Mutant fibulin-3 causes proteoglycan accumulation, reduction of MMP-2 and MMP-9, but increase of TIMP-3, and impairs diffusion across Bruch's membrane. PMID: 28622396
- Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560
- The Efemp1 knock-in mice reconstitute the most important histopathologic symptoms of both Malattia leventinese and age-related macular degeneration. PMID: 17664227
- fibulin-3 plays an important role in maintaining the integrity of fascia connective tissues and regulates aging. PMID: 17872905
- both fibulin-3 and -5 are important in maintaining pelvic organ support in mice. PMID: 19095964
- Data suggest a role for fibulin-3 in tissue maintenance and regeneration in the adult olfactory pathway. PMID: 19520159
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亞細(xì)胞定位：Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
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蛋白家族：Fibulin family
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數(shù)據(jù)庫鏈接：
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