Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit
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中文名稱：小鼠E3泛素連接酶SMURF1(SMURF1)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL021860MO
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠E3泛素連接酶SMURF1(SMURF1)酶聯(lián)免疫試劑盒（CSB-EL021860MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SMURF1含量。SMURF1即E3泛素連接酶，與細(xì)胞內(nèi)多條信號(hào)通路密切相關(guān)。它參與調(diào)控細(xì)胞生長、分化及胚胎發(fā)育等生理過程。研究機(jī)制上，其主要通過泛素化修飾底物蛋白，影響蛋白穩(wěn)定性和功能，在腫瘤、神經(jīng)退行性疾病等研究中受關(guān)注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外培養(yǎng)細(xì)胞或動(dòng)物模型中SMURF1蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測，為研究泛素化修飾機(jī)制、信號(hào)轉(zhuǎn)導(dǎo)調(diào)控及疾病相關(guān)分子網(wǎng)絡(luò)提供可靠工具，特別適合骨代謝研究、腫瘤微環(huán)境分析及藥物干預(yù)效果評(píng)估等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Smurf1 ELISA Kit; E3 ubiquitin-protein ligase SMURF1 ELISA Kit; EC 2.3.2.26 ELISA Kit; HECT-type E3 ubiquitin transferase SMURF1 ELISA Kit; SMAD ubiquitination regulatory factor 1 ELISA Kit; SMAD-specific E3 ubiquitin-protein ligase 1 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.8 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cell Biology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SMURF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-104 1:2 Average % 92 Range % 88-95 1:4 Average % 86 Range % 80-91 1:8 Average % 89 Range % 83-98 -
回收率：
The recovery of mouse SMURF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 101 94-109 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.804 2.600 2.702 2.535 1000 2.403 2.371 2.387 2.220 500 1.747 1.850 1.799 1.632 250 0.972 0.975 0.974 0.807 125 0.594 0.581 0.588 0.421 62.5 0.370 0.347 0.359 0.192 31.25 0.256 0.245 0.251 0.084 0 0.170 0.163 0.167 -
數(shù)據(jù)處理：
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貨期：3-5 working days
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相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
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基因功能參考文獻(xiàn)：
- Inhibition of osteoblastic Smurf1 promotes bone formation in mouse models of distinctive age-related osteoporosis PMID: 30143635
- Smurf1 expression in osteoblast differentiation is regulated by CRTC2. PMID: 30449452
- Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604
- Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690
- TGF-beta inhibited osteoblastic differentiation by inducing the MAPK-ERK pathway which upregulated the expression of ubiquitin ligase SMURF1 and resulted in reduced presence of osteogenic proteins. PMID: 28322449
- demonstration that Smurf1 acts as a brake for integrin activation by controlling Kindlin-2 protein levels, a new mechanism that permits precise modulation of integrin-mediated cellular functions PMID: 28408404
- miR-140-5p and SMURF1 are key regulators of disease pathology in pulmonary arterial hypertension PMID: 27214554
- Smurf1 is required for selective autophagy of Mycobacterium tuberculosis and host defense against tuberculosis infection. PMID: 28017659
- Smurf1 regulates glucose metabolism and inhibits osteoblast differentiation. Smurf1 function requires the presence of serine 148 (S148) in Smurf1. PMID: 27052174
- Smurf1 mediates NGF signaling and ubiquitinates RhoA in growth cones, regulating the need for local translation and degradation. PMID: 25901863
- Smurf-mediated endocytosis of Patched1 is required in sonic hedgehog signal reception PMID: 24925320
- Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199
- Smurf1-mediated Lys29-linked nonproteolytic polyubiquitination of axin1 negatively regulates Wnt/beta-catenin signaling. PMID: 23959799
- These results suggest that the JNK/AP-1 and ERK/Runx2 signaling pathways mediate TNF-alpha-dependent Smurf1 transcription. PMID: 23042144
- Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288
- studies uncover a cell-cycle-independent function of Cdh1, establishing Cdh1 as an upstream component that governs Smurf1 activity PMID: 22152476
- ER stress induces Smurf1 degradation and WFS1 up-regulation PMID: 21454619
- Smurf1 negatively regulates mesenchymal stem cell proliferation and differentiation by controlling JunB turnover through an ubiquitin-proteasome pathway. PMID: 20200942
- induction could trigger downregulation of bone morphogenetic protein signaling and cause vascular cell proliferation and remodeling in pulmonary arterial hypertension pulmonary arterial hypertension PMID: 20558834
- Smurf1 appears to be an important regulatory factor in osteoblast differentiation and a potential molecular target for identification of bone anabolic agents PMID: 12738770
- Smurf1 is required for the myogenic differentiation of C2C12 cells and plays an important regulatory role in the BMP-2-mediated osteoblast conversion PMID: 12871975
- Smurf1 plays a specific role in osteoblast differentiation and bone formation in vivo PMID: 14701828
- overexpression of Smurf1 specifically promotes Smad1 and Smad5 ubiquitination and degradation in embryonic lung epithelium PMID: 14711801
- Mating Smad6 and Smurf1 transgenic mice produced double-transgenic pups with more delayed endochondral ossification than Smad6 transgenic mice, suggesting that Smurf1 supports Smad6 function in vivo. PMID: 15123739
- Smurf1 negatively regulates osteoblast activity and response to BMP through controlling MEKK2 degradation. PMID: 15820682
- in chronic inflammatory disorders where TNF is increased, TNF induces the expression of ubiquitin ligase Smurf1 and promotes ubiquitination and proteasomal degradation of Smad1 and Runx2, leading to systemic bone loss PMID: 18567580
- the WW domains linker is important in complex assembly and in regulating activity of HECT-type E3s and CKIP-1 functions as the first auxiliary factor to enhance the activation of Smurf1. PMID: 18641638
- Mice mutant for Smurf1 and Smurf2 display planar cell polarity defects in the cochlea and convergence and extension movements defects that include a failure to close the neural tube. PMID: 19379695
- Initial identification of Smurf1 as an ubiquitin ligase that inhibits BMP signaling by targeting Smad1/5 for proteasomal destruction in cultured cells and Xenopus embryos. PMID: 10458166
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亞細(xì)胞定位：Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
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