Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

產(chǎn)品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠D-乳酸脫氫酶(D-LDH)酶聯(lián)免疫試劑盒（CSB-E11723m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的LDHD含量。試劑盒檢測范圍為4.69 mU/mL-300 mU/mL，適用于科研領(lǐng)域中對小鼠模型中D-LDH功能的研究，例如腸道缺血再灌注損傷機制探索、糖尿病相關(guān)代謝異常評估或細胞能量代謝實驗等場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Ldhd ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit; mitochondrial ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; EC 1.1.2.4 ELISA Kit
縮寫：

LDHD
Uniprot No.：

Q7TNG8
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

4.69 mU/mL-300 mU/mL
靈敏度：

1.17 mU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	93
	Range %	90-96
1:4	Average %	87
	Range %	83-90
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	104	101-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in D-lactate, but not L-lactate catabolic process.
基因功能參考文獻：
1. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
亞細胞定位：

Mitochondrion.
蛋白家族：

FAD-binding oxidoreductase/transferase type 4 family
組織特異性：

Readily detected in liver and kidney, with a weaker signal observed in heart, skeletal muscle, stomach, brain, and lung.
數(shù)據(jù)庫鏈接：

KEGG: mmu:52815

STRING: 10090.ENSMUSP00000068086

UniGene: Mm.271578