Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit
-
中文名稱：小鼠丙氨酸轉(zhuǎn)氨酶/谷丙轉(zhuǎn)氨酶(ALT/GPT)酶聯(lián)免疫試劑盒
-
貨號(hào)：CSB-E16539m
-
規(guī)格：96T/48T
-
價(jià)格：￥3800/￥2500
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：小鼠丙氨酸轉(zhuǎn)氨酶/谷丙轉(zhuǎn)氨酶(ALT/GPT)酶聯(lián)免疫試劑盒（CSB-E16539m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、細(xì)胞裂解物樣本中的ALT/GPT含量。ALT/GPT即谷丙轉(zhuǎn)氨酶，主要存在于肝細(xì)胞內(nèi)。肝細(xì)胞受損時(shí)其釋放入血，致血清ALT/GPT水平升高，是反映肝細(xì)胞損傷的敏感指標(biāo)。臨床常通過(guò)檢測(cè)其活性輔助診斷肝臟疾病，如肝炎、肝硬化等，研究也圍繞其在疾病中的變化機(jī)制展開(kāi)。試劑盒檢測(cè)范圍為0.156 mU/mL-10 mU/mL，廣泛應(yīng)用于肝損傷模型評(píng)估、藥物肝毒性研究、代謝性疾病機(jī)制探索等科研領(lǐng)域，特別適用于體外培養(yǎng)肝細(xì)胞或動(dòng)物模型中肝臟相關(guān)病理生理變化的動(dòng)態(tài)監(jiān)測(cè)，為肝臟功能研究提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
-
別名：Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
-
縮寫：ALT/GPT
-
Uniprot No.：
-
種屬：Mus musculus (Mouse)
-
樣本類型：serum, plasma, cell culture supernates, cell lysates
-
檢測(cè)范圍：0.156 mU/mL-10 mU/mL
-
靈敏度：0.039 mU/mL
-
反應(yīng)時(shí)間：1-5h
-
樣本體積：50-100ul
-
檢測(cè)波長(zhǎng)：450 nm
-
研究領(lǐng)域：Metabolism
-
測(cè)定原理：quantitative
-
測(cè)定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 83-95 1:2 Average % 97 Range % 92-101 1:4 Average % 106 Range % 98-113 1:8 Average % 94 Range % 87-97 -
回收率：
The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 88-97 EDTA plasma (n=4) 102 95-108 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mU/ml OD1 OD2 Average Corrected 10 2.645 2.566 2.606 2.421 5 2.015 1.855 1.935 1.750 2.5 1.194 1.155 1.175 0.990 1.25 0.777 0.796 0.787 0.602 0.625 0.492 0.461 0.477 0.292 0.312 0.335 0.344 0.340 0.155 0.156 0.278 0.288 0.283 0.098 0 0.191 0.178 0.185 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
引用文獻(xiàn)
- Biochemical Assessment of Hepatic and Renal Functions Following Administration of Doses of Monosodium Glutamate and Shilajit in Albino Mice E mahmoud Eltayef,research square,2025
- Andrographolide ameliorates sepsis-induced acute liver injury by attenuating endoplasmic reticulum stress through the FKBP1A-mediated NOTCH1/AK2 pathway J He, Z Huang, R Zou,Cell Biology and Toxicology,2025
- Targeting ATM enhances radiation sensitivity of colorectal cancer by Potentiating radiation-induced cell death and antitumor immunity Y Xie, Y Liu, M Lin, Z Li, Z Shen, S Yin, Y Zheng,Journal of Advanced Research,2024
- Gut microbiota dysbiosis induced by alcohol exposure in pubertal and adult mice J Yang, H Wang, X Lin, J Liu, Y Feng, Y Bai, H Liang,mSystems,2024
- Diosgenin Alleviates Obesity-Induced Insulin Resistance by Modulating PI3K/Akt Signaling Pathway in Mice Fed a High-Fat Diet SH Oh, MS Lee, BC Lee,Chemical and Pharmaceutical Bulletin,2024
- Influence of different types of dietary sugars on the intestinal mucosa and hepatic lipid metabolism in germ-free mice HK Son, H Jhun, HB Lee, YR Lee, M Park,Biochemical and Biophysical Research Communications,2024
- Thyme Extract Alleviates High-Fat Diet-Induced Obesity and Gut Dysfunction YR Lee,Nutrients,2023
- Narirutin-Rich Celluclast Extract from Mandarin (Citrus unshiu) Peel Alleviates High-Fat Diet-Induced Obesity and Promotes Energy Metabolism in C57BL/6 Mice ST Im,International journal of molecular sciences,2024
- Smart multi‐functional aggregates reoxygenate tumor microenvironment through a two‐pronged strategy to revitalize cancer immunotherapy Y Zhang,/,2024
- DNMT3B alleviates liver steatosis induced by chronic low-grade LPS via inhibiting CIDEA expression Q Li,Cellular and molecular gastroenterology and hepatology,2023
- Platelet-mimetic nano-sensor for combating postoperative recurrence and wound infection of triple-negative breast cancer Y Liu,Journal of controlled release,2023
- Testosterone Promotes the Intestinal Replication and Dissemination of Coxsackievirus B3 in an Oral Inoculation Mouse Model AH Dhalech,Journal of virology,2023
- β-arrestin 2 attenuates lipopolysaccharide-induced liver injury via inhibition of TLR4/NF-κB signaling pathway-mediated inflammation in mice Jiang MP,World journal of gastroenterology,2023
- Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance X Yan,Cellular and molecular life sciences,2024
- HCV Core Protein–ISX Axis Promotes Chronic Liver Disease Progression via Metabolic Remodeling and Immune Suppression LT Wang,Advanced science,2023
- Detection and Quantification of Some Ethanol-Producing Bacterial Strains in the Gut of Mouse Model of Non-Alcoholic Fatty Liver Disease: Role of Metformin M Abouelkheir,Pharmaceuticals,2023
- The Protective Effects of Corn Oligopeptides on Acute Alcoholic Liver Disease by Inhibiting the Activation of Kupffer Cells NF-κB/AMPK Signal Pathway Y Wei,Nutrients,2022
- Gellan gum prevents non-alcoholic fatty liver disease by modulating the gut microbiota and metabolites MH Do,Food chemistry,2022
- Sodium butyrate protects against lipopolysaccharide-induced liver injury partially via the GPR43/b-arrestin-2/NF-jB network QJ Luo,Gastroenterology report,2022
- Bifidobacterium animalis ssp. lactis MG741 Reduces Body Weight and Ameliorates Nonalcoholic Fatty Liver Disease via Improving the Gut Permeability and Amelioration of Inflammatory Cytokines MH Do,Nutrients,2022
- Corchorus olitorius L. ameliorates alcoholic liver disease by regulating gut-liver axis MH Do,Journal of Functional Foods,2021
- Quantitative Functional Evaluation of Liver Fibrosis in Mice with Dynamic Contrast-enhanced Photoacoustic Imaging J Lv,Radiology,2021
- Sodium butyrate protects against lipopolysaccharide-induced liver injury partially via the GPR43/β-arrestin-2/NF-κB network QJ Luo,Gastroenterology Report,2020
- Estrogen protects against liver damage in sepsis through inhibiting oxidative stress mediated activation of pyroptosis signaling pathway Z Xu,PLoS One,2020
- Hydrogen selenide, a vital metabolite of sodium selenite, uncouples the sulfilimine bond and promotes the reversal of liver fibrosis Dongrui Luan,SCIENCE CHINA Life Sciences,2020
- Inhibition of Inflammatory Responses by Centella asiatica via Suppression of IRAK1-TAK1 in Mouse Macrophages YC Cho,Am J Chin Med,2020
- T cells expressing a chimeric┸\PD1┸\Dap10┸\CD3zeta receptor reduce tumor burden in multiple murine syngeneic models of solid cancer G Parriott,Immunology,2020
- β-Arrestin1 enhances liver fibrosis through autophagy-mediated Snail signaling Siwei Tan.et al,faseb journal,2018
- Han.et al,Experimental and Therapeutic Medicine,2018
- Mingnuan Han.et al,Experimental and Therapeutic Medicine,2018
- A water-soluble nucleolin aptamer-paclitaxel conjugate for tumor-specific targeting in ovarian cancer Li F.et al,Nat Commun.,2017
- NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury Xiahou Z.et al,Nat Commun.,2017
- The Effects of Taoren-Honghua Herb Pair on Pathological Microvessel and Angiogenesis-Associated Signaling Pathway in Mice Model of CCl4-Induced Chronic Liver Disease Xi S.et al,Evidence-Based Complementary and Alternative Medicine,2016
- FTO-dependent function of N6-methyladenosine is involved in the hepatoprotective effects of betaine on adolescent mice Chen J. et al,J Physiol Biochem,2015
- Is plasma soluble CD36 associated with cardiovascular risk factors in early onset coronary artery disease patients? Krzystolik A et al,Scand J Clin Lab Invest,2015
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能：Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
-
基因功能參考文獻(xiàn)：
- Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
- PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
- In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
-
亞細(xì)胞定位：Cytoplasm.
-
蛋白家族：Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
-
組織特異性：Mainly expressed in liver, intestine, colon and white adipose tissue.
-
數(shù)據(jù)庫(kù)鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
