Mouse β-hexosaminidase A,β-Hex A ELISA Kit

Mouse β-hexosaminidase A,β-Hex A ELISA Kit

產(chǎn)品詳情

產(chǎn)品評價(jià)

靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品評價(jià)

相關(guān)產(chǎn)品

靶點(diǎn)詳情

Most popular with customers

產(chǎn)品中心

信號通路

技術(shù)信息

聯(lián)系信息

說明書下載

中文名稱：

小鼠β氨基己糖苷酶A(β-Hex A)酶聯(lián)免疫試劑盒
貨號：

CSB-E04818m
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品描述：

小鼠β氨基己糖苷酶A(β-Hex A)酶聯(lián)免疫試劑盒（CSB-E04818m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HEXA含量。HEXA是一種溶酶體酶，負(fù)責(zé)分解GM2神經(jīng)節(jié)苷脂，維持細(xì)胞內(nèi)環(huán)境穩(wěn)定。其突變會(huì)導(dǎo)致GM2神經(jīng)節(jié)苷脂在細(xì)胞內(nèi)積累，引發(fā)泰薩二氏病等疾病。研究HEXA的機(jī)制，旨在開發(fā)治療相關(guān)遺傳性神經(jīng)退行性疾病的藥物。試劑盒檢測范圍為3.12 μU/mL-200 μU/mL，適用于研究β-Hex A在代謝性疾病模型小鼠中的表達(dá)調(diào)控、基因敲除/過表達(dá)動(dòng)物模型的酶活性驗(yàn)證，以及藥物干預(yù)或病理?xiàng)l件下溶酶體功能的評估等科研場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Hexa ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexosaminidase subunit A ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit
縮寫：

HEXA
Uniprot No.：

P29416
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 μU/mL-200 μU/mL
靈敏度：

0.78 μU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	92
	Range %	85-100
1:20	Average %	93
	Range %	87-98
1:40	Average %	90
	Range %	86-100
1:80	Average %	90
	Range %	86-98

回收率：

The recovery of mouse β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-101
EDTA plasma (n=4)	95	92-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μU/ml	OD1	OD2	Average	Corrected
200	2.542	2.517	2.530	2.386
100	2.073	2.101	2.087	1.943
50	1.452	1.464	1.458	1.314
25	0.942	0.961	0.952	0.808
12.5	0.416	0.402	0.409	0.265
6.25	0.325	0.311	0.318	0.174
3.12	0.247	0.237	0.242	0.098
0	0.146	0.141	0.144

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A.
基因功能參考文獻(xiàn)：
1. Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice. PMID: 20862357
亞細(xì)胞定位：

Lysosome.
蛋白家族：

Glycosyl hydrolase 20 family
組織特異性：

Ubiquitous. Most abundant in testis, adrenal, epididymis and heart. Low levels seen in the liver.
數(shù)據(jù)庫鏈接：

KEGG: mmu:15211

STRING: 10090.ENSMUSP00000026262

UniGene: Mm.2284

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.