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中文名稱：人α2抗纖溶酶(α2-AP)酶聯免疫試劑盒
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貨號：CSB-E09452h
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規格：96T/48T
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價格：￥3200/￥2500
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其他：
產品詳情
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產品描述：人α2抗纖溶酶(α2-AP)酶聯免疫試劑盒（CSB-E09452h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的SERPINF2含量。SERPINF2是一個靶點。它在人體生理活動中有著重要意義。目前研究主要聚焦于其在細胞信號傳導、組織修復等方面的機制。深入探討它在相關疾病發生發展中扮演的角色，有望為研發針對性的治療藥物和策略提供理論支撐。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎醫學研究中纖溶系統功能評估、血栓相關疾病機制探索、肝臟合成功能研究及遺傳性纖溶異常疾病的體外分析，同時可為抗凝藥物開發提供可靠的生物標志物檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：A2AP ELISA Kit; A2AP_HUMAN ELISA Kit; AAP ELISA Kit; Alpha 2 antiplasmin ELISA Kit; Alpha 2 antiplasmin pigment epithelium derived factor ELISA Kit; Alpha 2 AP ELISA Kit; ALPHA 2 PI ELISA Kit; Alpha 2 plasmin inhibitor ELISA Kit; Alpha 2 plasmin inhibitor deficiency ELISA Kit; Alpha-2-antiplasmin ELISA Kit; Alpha-2-AP ELISA Kit; Alpha-2-PI ELISA Kit; Alpha-2-plasmin inhibitor ELISA Kit; Antiplasmin deficiency ELISA Kit; API ELISA Kit; Plasmin inhibitor deficiency ELISA Kit; PLI ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade F; member 2 ELISA Kit; Serine (Or cysteine) peptidase inhibitor; clade F; member 2; isoform CRA_c ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; Serine (Or cysteine) proteinase inhibitor; clade F; member 2 ELISA Kit; Serine or cysteine peptidase inhibitor clade F member 2 ELISA Kit; Serpin F2 ELISA Kit; Serpin peptidase inhibitor clade F ELISA Kit; Serpin peptidase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; SERPINF2 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma
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檢測范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Others
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human α2-AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 84-93 1:2 Average % 85 Range % 81-91 1:4 Average % 92 Range % 88-100 1:8 Average % 95 Range % 91-99 -
回收率：
The recovery of human α2-AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 83-94 EDTA plasma (n=4) 95 90-100 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1000 2.272 2.346 2.309 2.214 500 1.476 1.491 1.484 1.389 250 0.863 0.892 0.878 0.783 125 0.448 0.479 0.464 0.369 62.5 0.268 0.251 0.260 0.165 31.2 0.187 0.178 0.183 0.088 15.6 0.169 0.159 0.164 0.069 0 0.097 0.092 0.095 -
數據處理：
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貨期：3-5 working days
引用文獻
- Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome AH Mossa,World Journal of Urology,2020
產品評價
相關產品
靶點詳情
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功能：Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
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基因功能參考文獻：
- alpha2AP has a profibrotic effect probably not by the action as a plasmin inhibitor, and the blocking of alpha2AP exerts an antifibrotic effect in humans and mice with systemic sclerosis PMID: 26743600
- This review presents recent findings regarding the main aspects of the natural heterogeneity of A2AP with particular focus on the functional and possible clinical implications. [review] PMID: 26626994
- Possession of the alpha2AP 407Lys allele was negatively associated with AAA, and thus changes in alpha2AP may affect aneurysm growth and development. PMID: 25065555
- Changes in plasma A2AP are associated with cardiovascular disease event presentation. PMID: 26088309
- A significant correlation was found between soluble fibroblast activation protein levels and alpha2-antiplasmin cleavage in coronary thrombosis. PMID: 25464232
- increased N-terminal cleavage of alpha2AP may have a role in liver cirrhosis PMID: 24034420
- FXIII-A has a functional role through exposure on the activated platelet membrane where it exerts antifibrinolytic function by cross-linking alpha2AP to fibrin PMID: 25331118
- Data suggest serum A2AP (SERPINF2) level can serve as biomarker for diabetic retinopathy; levels of A2AP (but not fibrinogen, plasminogen, or plasminogen activator inhibitor 1) are up-regulated in hyperglycemic type 1 diabetes with retinopathy. PMID: 24818165
- protective mediator during gram-negative (pneumo)sepsis, limiting bacterial growth, inflammation, tissue injury, and coagulation PMID: 23992406
- Study revealed that plasmin was present in tumor tissue, and that it was responsible for processing progalanin to galanin(1-20) in the extracellular environment. PMID: 21707521
- Data suggest that decreased amounts of alpha2-plasmin inhibitor in plasma vs serum ex vivo may reflect reduced factor XIII (FXIII) in vivo; thus, plasma vs serum alpha2-plasmin inhibitor may be useful diagnostic marker for severe FXIII deficiency. PMID: 22205503
- fibrinolysis is enhanced by inhibiting enzymatic cleavage of precursor alpha2-antiplasmin PMID: 21251197
- Truncation of monocyte chemoattractant protein 1 by plasmin promotes blood-brain barrier disruption. PMID: 21486949
- Levels of free full-length alpha2AP were decreased in myocardial infarction (MI); the percentage of C-terminally cleaved alpha2AP was unaltered, and Arg407Lys did not influence alpha2AP levels or MI risk. PMID: 21505192
- the antifibrinolytic function of FXIII is independent of fibrin-fibrin cross-linking and is expressed exclusively through alphaAP. PMID: 21471521
- ADAMTS13 inactivation by plasmin as a potential cause of thrombotic thrombocytopenic purpura PMID: 20553378
- deficiency in patients with Philadelphia chromosome-positive haematologic cancer receiving imatinib mesylate PMID: 19492163
- OPN is highly susceptible to cleavage near its integrin-binding motifs, and the protein is a novel substrate for plasmin and cathepsin D PMID: 20071328
- FXIIIa substrate, A2AP, sequences suggests that the residue located two positions beyond the reactive glutamine is not conserved. this position makes important contributions to effective FXIIIa-A2AP interactions PMID: 19691486
- Multiple Lys residues within alpha 2-antiplasmin contribute, perhaps in a zipper-like fashion, to its binding to the in-tandem, multikringle array that configures the plasmin heavy chain. PMID: 12549929
- Alpha2-antiplasmin has an important role in acute pulmonary embolism PMID: 12911586
- the Arg6Trp polymorphism may play a significant role in governing the long-term deposition/removal of intravascular fibrin PMID: 17317851
- Fibrinolysis is amplified by converting alpha-antiplasmin from a plasmin inhibitor to a substrate PMID: 17883703
- Hydroxyethyl starch (HES) dilution enhances fibrinolysis by diminishing alpha2-antiplasmin-plasmin interactions. PMID: 17890952
- Sequencing analysis revealed the presence of two alpha2-PI gene variations, both in the second half of exon 10: a frameshift mutation and a G to A transition at nucleotide 11337 in codon 407. PMID: 17961166
- Thrombin activatable fibrinolysis inhibitor and alpha(2)-antiplasmin are not markers for recanalization in patients with ischemic stroke treated with recombinant tissue-type plasminogen activator. PMID: 18048863
- Plasmin in nephrotic urine activates the epithelial sodium channel PMID: 19073825
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相關疾病：Alpha-2-plasmin inhibitor deficiency (APLID)
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亞細胞定位：Secreted.
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蛋白家族：Serpin family
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組織特異性：Expressed by the liver and secreted in plasma.
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數據庫鏈接：
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