Human thyrotropin-releasing hormone,TRH ELISA KIT

說明書下載

中文名稱：

人促甲狀腺素釋放激素(TRH)酶聯免疫試劑盒
貨號：

CSB-E08146h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人促甲狀腺素釋放激素(TRH)酶聯免疫試劑盒（CSB-E08146h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的TRH含量。TRH即促甲狀腺激素釋放激素，它由下丘腦分泌，可刺激腺垂體分泌促甲狀腺激素。其背景在于它是調節甲狀腺功能的重要神經內分泌激素。研究機制主要圍繞其對甲狀腺軸的調控，及在神經系統中對神經遞質釋放、神經細胞生長分化等方面作用展開。試劑盒檢測范圍為2.5 pg/mL-100 pg/mL，本試劑盒適用于科研領域中對TRH表達水平的精準定量分析，可應用于神經內分泌調節機制研究、甲狀腺功能相關疾病模型建立、內分泌系統與代謝關聯性探索等方向。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TRH ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
縮寫：

TRH
Uniprot No.：

P20396
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

2.5 pg/mL-100 pg/mL
靈敏度：

1.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	95
	Range %	85-105
1:4	Average %	97
	Range %	92-102
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	91-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
100	2.232	2.252	2.242
50	1.196	1.156	1.176
25	0.635	0.648	0.642
12.5	0.357	0.362	0.360
5	0.146	0.151	0.149
2.5	0.075	0.079	0.077

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

As a component of the hypothalamic-pituitary-thyroid axis, it controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth. It promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes.
基因功能參考文獻：
1. TRH rs13097335 may have a protective role toward the development of breast cancer. PMID: 25362934
2. Data indicate that decrease of cholesterol level impaired the functional coupling between the thyrotropin-releasing hormone receptor and the cognate G proteins. PMID: 25485475
3. Cpe mutations in mouse may affect processing of prothyrotropin releasing hormone into thyrotropin releasing hormone. PMID: 12270926
4. TRH inhibits melanin-concentrating hormone (MCH) neurons by increasing synaptic inhibition from local GABA neurons. PMID: 22378876
5. This study introduces TRH as a novel, potent, selective, and evolutionarily highly conserved neuroendocrine factor controlling human pigmentation in situ. PMID: 21956127
6. Case Report: Isolated idiopathic central hypothyroidism in an adult, possibly caused by thyrotropin releasing hormone (TRH) deficiency. PMID: 20687402
7. Thus, human scalp hair follicles are both a source and a target of TRH, which operates as a potent hair-growth stimulator PMID: 19825978
8. TRH mRNA levels in "mute" thyrotropinomas: cause-effect or coincidence? PMID: 12067454
9. results show that that leptin can regulate trh gene expression via activation of intracellular signal transducer and activator of transcription 3 (STAT3) proteins in TRH neurons in the hypothalamus PMID: 14764629
10. The presence of TRH in dysplastic nevi may be predictive for the development of melanoma. PMID: 15328193
11. Our results support the hypothesis that patients with pseudohypoparathyroidism Ia have impaired sensitivity to both TSH and TRH. PMID: 18805917
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亞細胞定位：

Secreted.
蛋白家族：

TRH family
組織特異性：

Hypothalamus. Expressed in the hair follicle epithelium (at protein level).
數據庫鏈接：

HGNC: 12298

OMIM: 613879

KEGG: hsa:7200

STRING: 9606.ENSP00000303452

UniGene: Hs.182231

Human thyrotropin-releasing hormone,TRH ELISA KIT

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