Human thymus expressed chemokine,TECK ELISA Kit
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中文名稱：人胸腺表達(dá)趨化因子(TECK/CCL25)酶聯(lián)免疫試劑盒
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貨號：CSB-E09189h
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規(guī)格：96T/48T
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價格：￥3200/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人胸腺表達(dá)趨化因子(TECK/CCL25)酶聯(lián)免疫試劑盒（CSB-E09189h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL25含量。CCL25是一種趨化因子。它在免疫系統(tǒng)等方面有著重要背景，可參與調(diào)節(jié)白細(xì)胞遷移等過程。研究機(jī)制上，主要通過與相應(yīng)受體結(jié)合，激活細(xì)胞內(nèi)信號通路，進(jìn)而調(diào)控細(xì)胞行為，在炎癥、腫瘤等疾病研究中受關(guān)注。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，本試劑盒適用于科研領(lǐng)域的基礎(chǔ)研究，例如探究TECK/CCL25在免疫調(diào)節(jié)、黏膜免疫應(yīng)答中的分子機(jī)制，或用于評估實(shí)驗(yàn)?zāi)Ｐ椭性撘蜃釉诩膊“l(fā)生發(fā)展中的動態(tài)變化，為免疫相關(guān)疾病機(jī)制解析、藥物靶點(diǎn)篩選及生物標(biāo)志物研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：A130072A22Rik ELISA Kit; C-C motif chemokine 25 ELISA Kit; CCL 25 ELISA Kit; CCL25 ELISA Kit; CCL25_HUMAN ELISA Kit; Chemokine (C C motif) ligand 25 ELISA Kit; Chemokine (CC motif) ligand 25 ELISA Kit; Chemokine C C motif ligand 25 ELISA Kit; Chemokine CC motif ligand 25 ELISA Kit; Chemokine TECK ELISA Kit; Ck beta 15 ELISA Kit; Ckb 15 ELISA Kit; Ckb15 ELISA Kit; MGC125074 ELISA Kit; MGC150327 ELISA Kit; SCY A25 ELISA Kit; SCYA 25 ELISA Kit; SCYA25 ELISA Kit; Small inducible cytokine A25 ELISA Kit; Small inducible cytokine A25 isoform 1 precursor ELISA Kit; Small inducible cytokine A25 isoform 2 precursor ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 25 ELISA Kit; Small inducible cytokine subfamily A Cys Cys member 25 ELISA Kit; Small inducible cytokine subfamily A; member 25 ELISA Kit; Small-inducible cytokine A25 ELISA Kit; TECK ELISA Kit; TECKvar ELISA Kit; Thymus expressed chemokine ELISA Kit; Thymus-expressed chemokine ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：25 pg/mL-1600 pg/mL
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靈敏度：6.25 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TECK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 87-98 1:2 Average % 100 Range % 96-104 1:4 Average % 89 Range % 84-94 1:8 Average % 95 Range % 89-100 -
回收率：
The recovery of human TECK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 85-97 EDTA plasma (n=4) 104 97-110 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 1600 2.684 2.564 2.624 2.498 800 2.011 1.937 1.974 1.848 400 1.374 1.339 1.357 1.231 200 0.945 0.908 0.927 0.801 100 0.528 0.516 0.522 0.396 50 0.320 0.307 0.314 0.188 25 0.238 0.231 0.235 0.109 0 0.129 0.123 0.126 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
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基因功能參考文獻(xiàn)：
- CCL25 was able to induce proteoglycan and collagen type I production in anulus fibrosus-derived cells. PMID: 30060561
- It plays a pathogenic role in liver diseases and it will be a therapeutic target of the diseases. PMID: 27795503
- TECK was shown to be expressed by osteoblasts, and its receptor, CCR9, by osteoclast precursors. TECK increased P. gingivalis LPS-induced osteoclast numbers in an in vitro osteoclast formation assay using osteoclast precursors. T PMID: 26921718
- that CCL25/CCR9 signal may provide cancer cells with chemotactic abilities through influencing several epithelial-mesenchymal transitionmarkers PMID: 27008282
- CCR9/CCL25 interactions are not only involved in colitis pathogenesis but also correlate with colonic inflammatory burden; further supporting the existence of overlapping mucosal lymphocyte recruitment pathways between the inflamed colon and liver. PMID: 26873648
- CpG methylation is reduced in gingival biopsies of patients with periodontitis PMID: 26472015
- Studies indicate important roles played by chemokine ligand 25 (CCL25)/chemokine receptor 9 (CCR9) in tumorigenesis, tumor chemoresistance and metastasis. PMID: 26879872
- CCL25 mRNA and protein levels were significantly increased in the nasopharyngeal carcinoma group compared with the control group. PMID: 26279399
- CCR9-CCL25 interaction promoted proliferation and suppressed apoptosis of non-small cell lung cancer cells by activating the PI3K/Akt pathway. PMID: 25691296
- High CCL25 expression is associated with the pathogenesis of ulcerative colitis. PMID: 24936795
- Expression of CCR9 and CCL25, the only natural ligand of CCR9, was significantly higher (p<0.0001) in NSCLC tissues and serum respectively, compared to their respective controls. PMID: 25296976
- TECK derived from endometrial stromal cell and macrophages upregulates the number and function of Tregs in the ectopic milieu. PMID: 25275597
- CCL25 is an antimicrobial protein with bacteriocidal activity against E. coli and S. aureus. PMID: 12949249
- CCL25 and CCR9 regulate colorectal cancer progression and invasion. PMID: 22863617
- cell migration assays revealed that mesenchymal stromal cells (MSCs) have tropism toward multiple myeloma (MM) cells, and CCL25 was identified as a major MM cell-produced chemoattractant for MSCs. PMID: 22102554
- We provide the first evidence that CCR9 and its natural ligand CCL25 are highly expressed by ovarian cancer tissue and their expression correlates with histological subtypes. PMID: 21637913
- CCL25 enhanced the expression of MMP-1, -9, -11 and -13 active proteins by BrCa cells in a CCR9-dependent fashion. PMID: 21344163
- CCL25 may be involved in the differentiation of monocytes to macrophages particularly in rheumatoid arthritis PMID: 20738854
- over-expressed TECK interacts with CCR9 on the endometrial stromal cells in the endometriotic milieu, which may contribute to the onset and progression of endometriosis. PMID: 20081876
- demonstrate a unique pattern of regulation for CCL25 and suggest a role for caudal type homeo box proteins in regulating CCL25 transcription PMID: 16517733
- Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin alpha4beta1. PMID: 17510295
- High expression may explain high incidence of melanoma metastis to the small intestine. PMID: 18245522
- There was a robust migration of specific IgA- and IgM-antibody-secreting cells induced by Salmonella vaccination toward the mucosal chemokines CCL25 and CCL28 PMID: 19003934
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亞細(xì)胞定位：Secreted.
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蛋白家族：Intercrine beta (chemokine CC) family
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組織特異性：Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
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