Human thymic stromal lymphopoietin,TSLP ELISA Kit
產品詳情
-
產品描述：
人胸腺基質淋巴細胞生成素(TSLP)酶聯免疫試劑盒（CSB-E09316h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的TSLP含量。TSLP即胸腺基質淋巴細胞生成素，它是一種上皮細胞因子，在免疫系統中扮演重要角色。其能激活樹突狀細胞，調節T細胞分化，參與多種免疫反應。目前研究聚焦于它與哮喘、特應性皮炎等炎癥性疾病的關聯，有望成為相關疾病治療新靶點。試劑盒檢測范圍為0.312 ng/ml-20 ng/ml，適用于探索過敏反應機制、評估藥物干預效果或比較不同疾病模型中的TSLP動態變化。研究人員可通過該工具分析呼吸道或皮膚組織勻漿中TSLP的表達差異，研究免疫細胞激活過程中血漿/血清生物標志物的波動規律，或驗證基因編輯/抑制劑處理對靶蛋白分泌的影響，為免疫調節研究和炎癥性疾病機制解析提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
-
別名：
Thymic stromal lymphopoietin ELISA Kit; Thymic stromal lymphopoietin protein TSLP ELISA Kit; Tslp ELISA Kit; TSLP protein ELISA Kit; TSLP_HUMAN ELISA Kit
-
縮寫：
-
Uniprot No.：
-
種屬：
Homo sapiens (Human)
-
樣本類型：
serum, plasma, tissue homogenates.
-
檢測范圍：
0.312 ng/ml-20 ng/ml
-
靈敏度：
0.078 ng/ml
-
反應時間：
1-5h
-
樣本體積：
50-100ul
-
檢測波長：
450 nm
-
研究領域：
Immunology
-
測定原理：
quantitative
-
測定方法：
Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
-
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TSLP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| Sample | Serum(n=4) | |
1:1 | Average % | 90 | |
Range % | 84-97 | |
1:2 | Average % | 92 | |
Range % | 86-99 | |
1:4 | Average % | 91 | |
Range % | 84-98 | |
1:8 | Average % | 93 | |
Range % | 86-98 | |
-
回收率：
The recovery of human TSLP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 95 | 89-98 | |
EDTA plasma (n=4) | 92 | 86-97 | |
-
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| | | | | | |
ng/ml | OD1 | OD2 | Average | Corrected | |
20 | 3.002 | 2.934 | 2.968 | 2.786 | |
10 | 1.935 | 1.867 | 1.901 | 1.719 | |
5 | 1.163 | 1.128 | 1.146 | 0.964 | |
2.5 | 0.734 | 0.729 | 0.732 | 0.550 | |
1.25 | 0.406 | 0.421 | 0.413 | 0.231 | |
0.625 | 0.306 | 0.293 | 0.299 | 0.118 | |
0.312 | 0.246 | 0.254 | 0.250 | 0.068 | |
0 | 0.179 | 0.185 | 0.182 | | |
-
本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human TSLP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TSLP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
顯示更多
收起更多
-
本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
顯示更多
收起更多
-
數據處理：
-
貨期：
3-5 working days
靶點詳情
-
功能：
Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.; May act as an antimicrobial peptide in the oral cavity and on the skin.
-
基因功能參考文獻：
- TSLP receptor allosterically activates TSLP to potentiate the recruitment of the shared interleukin 7 receptor alpha-chain (IL-7Ralpha) by leveraging the flexibility, conformational heterogeneity and electrostatics of the cytokine. PMID: 28368013
- Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells. PMID: 30011850
- presence of epithelium-derived TSLP in the eustachian tubes plays important role in the onset of eosinophilic otitis media PMID: 29272985
- a new mechanism of T cell stimulation in human skin was identified, demonstrating the direct induction of T cell migration by TSLP in the absence of DCs. PMID: 28377574
- the secretion of TSLP by keratinocytes is induced by the HIV-1 viral synapse in a miR-375 controlled manner PMID: 28443609
- production increased at skin sites of ovalbumin sensitization PMID: 27992078
- Sera of patients with infantile eosinophilic gastroenteritis showed a specific increase in both thymic stromal lymphopoietin and IL-33 levels. PMID: 26948075
- short and long thymic stromal lymphopoietin isoforms have roles in house dust mite-induced asthmatic airway epithelial barrier disruption PMID: 27996052
- DeltaNp73 was abundantly expressed in the atopic dermatitis epidermis and increased the release of TSLP via NF-kappaB activation. PMID: 28655470
- this study shows that long form of thymic stromal lymphopoietin of keratinocytes is induced by protein allergens PMID: 28720058
- we found that activation of the protease-activated receptor 1 (PAR1) induced secretion of TSLP by the corneal stromal cells... we proposed that TSLP might function as the link between increased protease activity and inflammatory responses or itch sensation in the corneas PMID: 28631887
- TSLP-HOTAIR axis also plays a protective role in low-density lipoprotein (ox-LDL)-induced Endothelial cells' injury. PMID: 28615347
- The aim of this study was to determine the influence of human rhinovirus on the genes involved in airway remodeling and to examine the impact of TSLP and contribution of oxidative stress on airway remodeling in the context of HRV infection. PMID: 28545810
- TSLP-induced phosphorylation of l-plastin is increased in atopic dermatitis and increases eosinophil migration. PMID: 27304220
- a hit expansion exercise yielded additional active compounds that could be amenable to further optimization, providing an opportunity to dissociate TSLP inhibition from other non-desired activities. This study illustrates the potential of phenotypic drug discovery to complement target based approaches by providing new chemistry and biology leads PMID: 29320511
- study found that among patients with atopic dermatitis (AD), common FLG null mutations are associated with earlier AD onset in a dose-dependent manner, whereas TSLP rs1898671 appears unrelated to the timing of AD onset PMID: 28479194
- lactobacilli from the omnivorous group and all bifidobacteria significantly down-regulated IL-8. Notably, both genera also lowered the TSLP expression in stimulated Caco-2 cells, regardless of the diet regimen. PMID: 27863334
- this review focuses on recent advances on the effects of TSLP on obstetrical and gynecological diseases, including pregnancy failure, endometriosis, and cervical cancer PMID: 27976427
- The increase in TSLP and TNF-alpha level observed in IS fluid was found to correlate with disease severity. The increased TSLP production from asthma sputum cells was abrogated by the addition of rIL-37. Regulation of TSLP pathway may be a therapeutic approach for asthma. PMID: 27528425
- House dust mite sublingual immunotherapy downregulated Th2-type immune responses mediated by the TSLP-OX40L signaling pathway in patients with persistent moderate to severe allergic rhinitis. PMID: 27012942
- The results suggest that IL-1beta may be an early key mediator for the acquisition of an Atopic dermatitis (AD) phenotype through induction of thymic stromal lymphopoietin (TSLP) and alteration of the epidermal homeostasis. PMID: 28191908
- House dust mite exposure increases the expression of TSLP through PI3K/Akt signaling pathway in human bronchial epithelial 16HBE cells. PMID: 28400057
- Posttranslational modifications control the functional activity of TSLP in humans and overproduction of TSLP may be a key trigger for the amplification of type 2 inflammation in diseases PMID: 27744031
- Study provides evidence for the hypothesis that TSLP could reflect the histological severity of lumbar disc degeneration (LDD), and TSLP-siRNA and TSLPR-siRNA could inhibit apoptosis of nucleus pulposus cells. The evident information obtained from the investigation could lead the way for new therapeutic approaches regarding LDD treatment. PMID: 28746197
- periostin-mediated TSLP production by keratinocytes directly stimulates cutaneous T-cell lymphoma (CTCL) tumor cell growth in addition to inducing a Th2-dominant tumor environment in CTCL. PMID: 27634769
- this study shows that TSLP may be involved in the pathogenesis of asthma and rhinitis; dust mite and mugwort allergy could increase it significantly PMID: 28303765
- Serum TSLP levels were significantly increased in systemic sclerosis patients compared to healthy donors, and were associated with a higher frequency of vasculopathy. The proportion of TSLP-positive dermal cells was increased in the skin of SSc patients compared with healthy donors, and was correlated with fibrosis. PMID: 27429171
- this study shows that NOD2 is an asthma-related factor that can promote cell proliferation and inflammatory response by mediated expression of TSLP in human airway smooth muscle cells PMID: 27889082
- the effects of TSLP on myeloid cells are crucial in reducing the multiple organ failure that is associated with systemic inflammation. PMID: 26934097
- interactions between TSLP in follicular dendritic cells and IgA production in tonsils may be an important mechanism contributing to the pathogenesis of Immunoglobulin A (IgA) nephropathy. PMID: 27187742
- Study demonstrated that naturally occurring severe infections by the most common respiratory viruses in hospitalized infants induce nasal airway secretion of TSLP, IL-33, and periostin when compared with healthy controls PMID: 28471975
- Variations in FLG and TSLP genotype were associated with differences in self-reported skin clearance, TCI usage, and steroid usage. PMID: 27902816
- A possible role of TSLP in immune homeostasis in HIV infection:higher plasma TSLP was found in individuals with primary HIV infection compared to both chronic HIV infection and healthy controls. PMID: 27769179
- The polymorphism was further reproducibly associated with a DNA methylation signature in whole blood (P = 4.5x10-40) that also associated with allergic sensitization and expression in blood of the cytokine TSLP (P = 1.1x10-4). Knockdown of the transcription factor predicted to bind the enhancer region (NHLH1) in a human cell line (HEK293) expressing NHLH1 resulted in lower TSLP expression PMID: 27149122
- TSLP level was significantly higher in asthmatic than in nonasthmatic children. TSLP level was significantly different between allergic asthmatic and nonallergic nonasthmatic groups. PMID: 26999524
- While serum TSLP levels were unaffected by concomitant allergies and atopic comorbidities, serum levels of IL-31, IL-33 and sST2 were affected to a small extent. We found a positive correlation between TSLP, IL-31 and IL-33, and an inverse relationship between IL-33 and sST2 PMID: 27152943
- Results showed that TSLP promoted the production of alpha-SMA and collagen I (P<0.001), suggesting that it can accelerate MRC-5 cell fibrosis. PMID: 27385084
- TSLP is a key mediator in the pathogenesis of inflammatory bowel diseases and that further studies are needed to evaluate its role. [review] PMID: 27697608
- Letter: report decreased salivary gland TSLP expression in primary Sjogren's syndrome. PMID: 27494755
- A novel mouse xenograft model to study the role of TSLP-induced CRLF2 signals in normal and malignant human B lymphopoiesis has been developed. PMID: 26611474
- Heat stress-induced HSPs can significantly reduce the production and secretion of TSLP from keratinocytes cultured under Th2 environment. PMID: 26419317
- TSLP expression in the skin is mediated via RARgamma-RXR pathways. PMID: 26531761
- Epidermal thymic stromal lymphopoietin predicts the development of atopic dermatitis during infancy. PMID: 26879860
- study shows that specific food antigens can trigger innate immune mediated esophageal TSLP secretion PMID: 26992000
- production by monocyte-derived dendritic cells requires the integration of signals from dectin-1, the IL-1 receptor, and endoplasmic reticulum stress response stress signaling pathways PMID: 26573878
- The association of TSLP polymorphism rs2289278 with atopic dermatitis (AD) was stronger in children with allergic sensitization than in children without atopy, and TSLP polymorphisms also increased the risk of asthma in children with AD PMID: 26712523
- The positive feedback loop between TSLP, IL-33 and their receptors, and Th2 cytokines may facilitate Th2-skewed inflammation in eosinophilic CRSwNP. PMID: 26095319
- results show that rs1837253 polymorphism may be directly involved in the regulation of TSLP secretion. PMID: 25515628
- Upon TSLP stimulation, the cells with the highest TSLPR expression level showed enhanced proliferation and JAK/STAT-mediated gene regulation in a dose-dependent manner PMID: 26652578
- Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent PMID: 25962901
顯示更多
收起更多
-
亞細胞定位：
Secreted.
-
組織特異性：
Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva.
-
數據庫鏈接：
Most popular with customers
