Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2) ELISA kit

Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2) ELISA kit

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中文名稱：

人蛋白酶體（prosome，macropain）亞基，β型，2(PSMB2)酶聯免疫試劑盒
貨號：

CSB-E17836h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人蛋白酶體（prosome，macropain）亞基，β型，2(PSMB2)酶聯免疫試劑盒（CSB-E17836h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PSMB2含量。PSMB2 是一個重要靶點。它在多種生理和病理過程中發揮作用，可能與細胞內蛋白降解、免疫調節等機制相關。對其研究有助于深入了解疾病發生發展的分子機制，為開發針對該靶點的藥物，治療相關疾病提供理論依據。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于基礎科研中探究蛋白酶體功能調控機制、腫瘤發生發展相關蛋白代謝異常、神經退行性疾病病理模型中的蛋白穩態失衡等研究領域；可高效支持體外培養細胞、動物模型組織或體液樣本中PSMB2的定量分析，為研究蛋白質降解通路在疾病發生中的作用或藥物干預效果評價提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

HC7 I ELISA Kit; Macropain subunit C7 I ELISA Kit; Macropain subunit C7-I ELISA Kit; Multicatalytic endopeptidase complex subunit C7 1 ELISA Kit; Multicatalytic endopeptidase complex subunit C7 I ELISA Kit; Multicatalytic endopeptidase complex subunit C7-I ELISA Kit; Proteasome (prosome, macropain) subunit beta type 2 ELISA Kit; Proteasome beta 2 subunit ELISA Kit; Proteasome component C7 I ELISA Kit; Proteasome component C7-I ELISA Kit; Proteasome subunit beta type-2 ELISA Kit; PSB2_HUMAN ELISA Kit; Psmb2 ELISA Kit
縮寫：

PSMB2
Uniprot No.：

P49721
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.2 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PSMB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	99-108
1:2	Average %	86
	Range %	83-89
1:4	Average %	94
	Range %	89-98
1:8	Average %	93
	Range %	88-97

回收率：

The recovery of human PSMB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	88	82-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.750	2.852	2.801	2.656
800	2.048	2.147	2.098	1.953
400	1.285	1.383	1.334	1.189
200	0.763	0.785	0.774	0.629
100	0.416	0.424	0.420	0.275
50	0.251	0.262	0.257	0.112
25	0.204	0.210	0.207	0.062
0	0.144	0.145	0.145

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Identification of a Novel Serum Biomarker for Tuberculosis Infection in Chinese HIV Patients by iTRAQ-Based Quantitative Proteomics Chen C,Frontiers in microbiology,2023

功能：

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
基因功能參考文獻：
1. Results showed that the overexpression of beta2 subunit decreased homologous recombination in human cells without altering the cell proteasome activity and the Rad51p level. PMID: 21660142
2. This subunit propeptides influence cooperative proteasome assembly. PMID: 12456675
亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Peptidase T1B family
數據庫鏈接：

HGNC: 9539

OMIM: 602175

KEGG: hsa:5690

STRING: 9606.ENSP00000362334

UniGene: Hs.471441

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.