Human platelet-derived growth factor CC (PDGF-CC) ELISA Kit
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中文名稱：人血小板衍生生長(zhǎng)因子CC(PDGF-CC)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E13241h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人血小板衍生生長(zhǎng)因子CC(PDGF-CC)酶聯(lián)免疫試劑盒（CSB-E13241h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的PDGF-CC含量。PDGF-CC 即血小板衍生生長(zhǎng)因子 CC，在細(xì)胞生長(zhǎng)、增殖等生理過(guò)程中發(fā)揮作用。其異常表達(dá)與腫瘤、纖維化等疾病的發(fā)生發(fā)展相關(guān)。研究機(jī)制主要聚焦于其信號(hào)通路，阻斷該信號(hào)有望為相關(guān)疾病治療提供方向，目前是研究熱門(mén)靶點(diǎn)。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，科研人員可通過(guò)該產(chǎn)品開(kāi)展PDGF-CC在疾病模型中的表達(dá)水平監(jiān)測(cè)、藥物干預(yù)效果評(píng)估或信號(hào)通路機(jī)制研究，為心血管生物學(xué)、腫瘤微環(huán)境及再生醫(yī)學(xué)等領(lǐng)域的體外實(shí)驗(yàn)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：PDGF-CC
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種屬：Homo sapiens (Human)
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樣本類型：serum, cell culture supernates, urine, tissue homogenates
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檢測(cè)范圍：0.156 ng/mL-10 ng/mL
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靈敏度：0.039 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cardiovascular
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 95 Range % 92-99 1:4 Average % 94 Range % 89-101 1:8 Average % 89 Range % 84-95 -
回收率：
The recovery of human PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-102 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 10 2.308 2.418 2.363 2.260 5 1.887 2.032 1.960 1.857 2.5 1.452 1.423 1.438 1.335 1.25 0.782 0.821 0.802 0.699 0.625 0.477 0.497 0.487 0.384 0.312 0.325 0.346 0.336 0.233 0.156 0.215 0.205 0.210 0.107 0 0.104 0.101 0.103 -
數(shù)據(jù)處理：
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貨期：3-5 working days
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