• A 96-well Assay plate --The 96-well plate has been pre-coated with PTH 1-34.
• Negative Control (1 x 800 μl) --Eliminate false positive
• Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of experimental results.
• Sample Diluent (2x 20 ml) --Dilute the sample to an appropriate concentration.
• HRP-conjugated Immunoglobulin (1 x 10ml)--Bind to the PTH 1-34 antibody and HRP catalyzes the TMB to elicit a chromogenic reaction.
• HRP-conjugate Diluent (2x 20 ml) --(1 x 20ml)
• Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
• Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
• TMB Substrate (1 x 10ml) --Interact with HRP, eliciting a Chromogenic reaction.
• Stop Solution (1 x 10 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells)--Cover the microplate when incubating.
• An Instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

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