Human mucin-5AC (MUC5AC) ELISA kit
產(chǎn)品詳情
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產(chǎn)品描述：
人粘蛋白5AC(MUC5AC)酶聯(lián)免疫試劑盒（CSB-E10109h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液樣本中的MUC5AC含量。MUC5AC是重要的黏蛋白基因，其編碼的黏蛋白在呼吸道等部位黏膜表面起保護(hù)屏障作用。但過度表達(dá)會堵塞氣道，與哮喘、慢阻肺等呼吸道疾病發(fā)生發(fā)展密切相關(guān)。研究多聚焦于抑制其表達(dá)及調(diào)控機(jī)制，以探索治療相關(guān)疾病的新靶點(diǎn)。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，科研人員可通過該產(chǎn)品探究呼吸道炎癥模型中黏液分泌調(diào)控機(jī)制、評估腫瘤微環(huán)境中黏蛋白的表達(dá)特征，或結(jié)合尿液樣本分析泌尿系統(tǒng)相關(guān)疾病中 MUC5AC 的潛在生物學(xué)意義。為分子機(jī)制探索及生物標(biāo)志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
MUC5AC ELISA Kit; MUC5 ELISA Kit; Mucin-5AC ELISA Kit; MUC-5AC ELISA Kit; Gastric mucin ELISA Kit; Major airway glycoprotein ELISA Kit; Mucin-5 subtype AC ELISA Kit; tracheobronchial ELISA Kit; Tracheobronchial mucin ELISA Kit; TBM ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, cell culture supernates, urine
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檢測范圍：
15.6 ng/mL-1000 ng/mL
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靈敏度：
3.9 ng/mL
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Signal Transduction
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 87-99 | |
| 1:2 | Average % | 94 | |
| Range % | 87-96 | |
| 1:4 | Average % | 98 | |
| Range % | 94-106 | |
| 1:8 | Average % | 90 | |
| Range % | 86-97 | |
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回收率：
| The recovery of human MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 95-93 | |
| EDTA plasma (n=4) | 94 | 91-97 | |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.452 | 2.563 | 2.508 | 2.375 | | | 500 | 1.949 | 1.964 | 1.957 | 1.824 | | | 250 | 1.358 | 1.431 | 1.395 | 1.262 | | | 125 | 0.812 | 0.832 | 0.822 | 0.689 | | | 62.5 | 0.536 | 0.576 | 0.556 | 0.423 | | | 31.2 | 0.342 | 0.356 | 0.349 | 0.216 | | | 15.6 | 0.241 | 0.249 | 0.245 | 0.112 | | | 0 | 0.132 | 0.134 | 0.133 | | |
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本試劑盒所含材料：
- A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human MUC5AC antibody.This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labled MUC5AC antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻(xiàn)
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Targeted Inhibition of Select ERK1/2 Functions Mitigates Pathological Features of Asthma in Mice
SD Shah,American journal of respiratory cell and molecular biology,2023
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Gleditsia sinensis Lam. aqueous extract attenuates nasal inflammation in allergic rhinitis by inhibiting MUC5AC production through suppression of the STAT3/STAT6 pathway
MA Jung,Biomedicine & pharmacotherapy,2023
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Understanding the Clinical Significance of MUC5AC in Biliary Tract Cancers
KK Benson,Cancers,2023
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Transcriptome Analysis Reveals Immunomodulatory Effect of Spore-Displayed p75 on Human Intestinal Epithelial Caco-2 Cells
SJ Kang,International journal of molecular sciences,2022
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IL-19 induces MUC5AC overproduction in hepatolithiasis via stat3 pathway
X WU,researchsquare,2022
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Effects of Spore-Displayed p75 Protein from Lacticaseibacillus rhamnosus GG on the Transcriptional Response of HT-29 Cells
SJ Kang,Microorganisms,2022
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Molecular mechanism underlying miR-130b-Sp1 transcriptional regulation in LPS-induced upregulation of MUC5AC in the bile duct epithelium
X Wu,Molecular medicine reports,2020
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Evaluation of serum MUC5AC in combination with CA19-9 for the diagnosis of pancreatic cancer
Zhang J,World J Surg Oncol,2020
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Caveolin-1 aggravates cigarette smoke extract-induced MUC5AC secretion in human airway epithelial cells
Yu Q et al,Int J Mol Med,2015
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Biodegradable nanoparticles meet the bronchial airway barrier: how surface properties affect their interaction with mucus and epithelial cells
/,Biomacromolecules,2011
靶點(diǎn)詳情
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功能：
Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD).
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基因功能參考文獻(xiàn)：
- S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID: 29906464
- The results of the present study indicate that Munc132 may be an essential regulator of basal MUC5AC exocytosis, while Munc134 appears to be a Munc13 protein subtype that may to be sensitive to hNE stimulation during airway MUC5AC hypersecretion. PMID: 29767240
- resistin induced MUC5AC and MUC5B expression via activation of different signaling pathways in human airway epithelial cells. PMID: 29604272
- the splice switch to DeltaC-ZIP2 as well as decreased expression of other ZIPs caused zinc deficiency, which is sufficient for induction of MUC5AC. PMID: 29289532
- this paper shows that IL-1beta upregulates Muc5ac expression via NF-kappaB-induced HIF-1alpha in asthma PMID: 29031476
- Data suggest that secreted IL-6 regulates CLB2.0-induced MUC5AC and MUC1 expression via ROS-mediated downregulation of claudin-1 expression to maintain mucus homeostasis in the airway. PMID: 28946937
- Helicobacter pylori (H. pylori)-CagA significantly upregulated MUC5AC, MUC2, and MUC5B expression in AGS cells, but did not affect E-cadherin and MUC6 expression. PMID: 29869461
- Oct-4 expression and the reduction of MUC5AC expression may be involved in the progression and an unfavorable prognosis of GC PMID: 28762513
- smoking might decrease tear secretion, goblet cell density and tear MUC5AC concentration. PMID: 27297822
- High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells PMID: 29286856
- Cyclic pressure did not induce MUC5AC secretion in the airway mucus epithelium via Ca(2+)-dependent ATP release, and nearly all Ca(2+) was provided by stored intracellular Ca(2). PMID: 27919041
- IL-33 induced MUC5AC mRNA and MUC5AC protein, and also goblet cell hyperplasia at air liquid interface culture in human nasal epithelial cells. In addition to that, IL-33 induced MUC5B and FOXA3, and reduces FOXJmRNA. PMID: 27776277
- MUC5AC has pathologic roles in mechanisms of allergen-induced airway hyperresponsiveness, mucous metaplasia, and airway mucus plugging. PMID: 27845589
- Leptin knockdown suppressed MUC5AC production and secretion induced by IL-13 in human bronchial epithelial cells. PMID: 28942146
- The results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating sessile serrated adenomas/polyps (SSA/Ps) from hyperplastic polyps (HPs). PMID: 28430953
- The Egr-1 is essential for CSE-induced MUC5AC production in HBE cells likely through interaction with and modulation of AP-1, and re-emphasize targeting Egr-1 as a novel therapeutic strategy for COPD. PMID: 28602698
- Mucus plugs from individuals with fatal asthma are heterogeneous gels with distinct MUC5AC- and MUC5B-containing domains. Tethering of MUC5AC-containing domains to the epithelium causes mucostasis and likely represents a major cause of mucus plugging in asthma. PMID: 27183390
- The only individual marker that was found to have direct and strong correlation with the clinical outcome of ampullary carcinoma was MUC5AC (not used in the Ang or Chang panels), with statistically significant survival differences found with various cutoffs tested (for 20% cutoff, 5-y survival, 68% vs. 31%; P=0.0002). In addition, MUC5AC significantly stratified the histologically pancreatobiliary and intestinal cases. PMID: 28505002
- MUC5AC interacts with integrin beta4 that mediates phosphorylation of FAK at Y397 leading to lung cancer cell migration. PMID: 26751774
- Results show that the glycoforms of MUC5AC in mucinous cysts of the pancreas display two unusual motifs, terminal alphaGlcNAc and terminal betaGlcNAc. PMID: 27992432
- MUC5AC expression was significantly higher in pancreatic adenocarcinoma than in healthy controls and chronic pancreatitis patients. PMID: 27845339
- Cigarette smoke exposure initially triggers the production of mitoROS and subsequently elicits autophagy in airway epithelial cells, which then regulate MUC5AC expression partially through modulation of the JNK signaling and the transcription factor AP-1. PMID: 27084849
- this study shows that S-allylmercapto-l-cysteine can suppress MUC5AC secretion using the cell model of chronic obstructive pulmonary disease PMID: 27517516
- This paper aims at developing a quantitative computer-aided methodology for automated identification of mucin areas and its percentage using tissue histological images PMID: 26971129
- MUC5B was significantly more often detected in middle ear effusion fluid relative to MUC5AC. MUC5AC presence was not significantly different in mucoid and serous fluid. PMID: 27729120
- outcome, while the gain of aberrant expression of MUC5AC and particularly of MUC6 was associated with favorable outcome in colorectal cancer, notably in intermediate stages II and III PMID: 27298226
- Low mucin1 expression is associated with peritoneal dissemination of gastrointestinal cancer. PMID: 27193208
- The HNE-TACE signalling pathway has an important role in the process of MUC5AC overexpression in chronic rhinosinusitis. PMID: 26881964
- Knockdown of MUC5AC in SW620 cells remarkably suppressed cell vitality and promoted apoptosis and G1 cell cycle arrest, resulting in the impaired ability of colony formation. The inhibition of MUC5AC in SW620 cells dramatically repressed the cell migration and invasion. PMID: 27610469
- These results suggest that IFN-gamma represses MUC5AC expression, disturbing binding of Sp1 to its target sequences. PMID: 27324793
- this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells PMID: 26980390
- The interference of p38 gene expression inhibited the release of IL-1beta and TNF-alpha in cultured cells. It also depressed both mRNA and protein levels of MUC5A. PMID: 26722604
- The expression of messenger RNAs is inhibited by EGFR tyrosine kinase inhibitor PMID: 26867523
- is a major component of the mucus produced by airway epithelial cells. PMID: 26100173
- Review-Meta-analysis: decreased Muc5AC expression might be a poor prognostic predictor for gastric cancer. PMID: 26420972
- Data show that verproside inhibits tumor necrosis factor alpha-induced mucin 5AC (MUC5AC) expression through NF-kappa B pathway. PMID: 26318254
- Overexpression of MUC5AC has been described in idiopathic pulmonary fibrosis lungs. Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201507-1322LE#.V2WAGNLrtNs PMID: 26871672
- The expression of ALPi and MUC5AC in cocultures of Caco-2 and HT29 cells developed for permeability studies is reported. PMID: 26299896
- MUC5AC hypomethylation is predictive of serrated lesions with malignant potential PMID: 26476272
- EGF removal or tyrphostin AG1478 treatment of differentiating air-liquid interface cultures from asthmatic children would result in a reduction of epithelial goblet cells and mucus secretion. PMID: 26057128
- These data lend support to the notion that beta2AR-agonists may contribute to asthma exacerbations by increasing mucin production via activation of beta2ARs on epithelial cells. PMID: 26161982
- The measurement of the CA 19-9 antigen on individual proteins could improve performance over the standard CA 19-9 assay. PMID: 22220206
- Studied the effects of 15-HETE on MUC5AC expression and related pathways. PMID: 25649981
- Increased expression of MUC5AC was associated with bacterial biofilm formation in chronic rhinosinusitis patients. PMID: 25638393
- Leukotriene E4 induces MUC5AC release from human airway epithelial NCI-H292 cells PMID: 25838093
- Results showed that MUC5AC expression is correlated to the tumor stage where advanced gastric cancers present reduced levels of MUC5AC and that Gli regulates its expression via interaction with a highly conserved sequence in its promoter region. PMID: 25166306
- MUC5AC expression in ovarian mucinous tumors did not change from benign to malignant tumors. PMID: 25298197
- Common genetic variations in the MUC5AC gene are not related to helicobacter pylori serologic status. PMID: 25605164
- The genetic length variants in the central repetitive region of MUC5AC were not associated with meconium ileus in cystic fibrosis. PMID: 24920497
- Data suggest that determination of mucin 5AC (MUC5AC) methylation status may be useful for understanding and predicting the natural history of colorectal cancers (CRC). PMID: 25403854
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亞細(xì)胞定位：
Secreted.
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組織特異性：
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum.
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