Human guanylate cyclase activator 2B (GUCA2B) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人鳥苷酸環化酶激活劑2B (GUCA2B)酶聯免疫試劑盒（CSB-EL010048HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織培養上清液樣本中的GUCA2B含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于基礎科研領域中對胃腸疾病、腎臟生理及代謝綜合征等相關機制的研究，為探究GUCA2B在體液平衡調控及相關病理過程中的分子功能提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GUCA2B ELISA Kit; GCAP II ELISA Kit; GCAP-II ELISA Kit; Guanylate cyclase activator 2B (uroguanylin) ELISA Kit; Guanylate cyclase activator 2B ELISA Kit; Guanylate cyclase C-activating peptide II ELISA Kit; GUC2B_HUMAN ELISA Kit; GUCA2B ELISA Kit; UGN ELISA Kit; Uroguanylin ELISA Kit
縮寫：

GUCA2B
Uniprot No.：

Q16661
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, cell culture supernates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
Range %	85-91
1:10	Average %	102
Range %	98-105
1:20	Average %	99
Range %	93-105
1:40	Average %	95
Range %	92-97

回收率：

The recovery of human GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-96
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.
基因功能參考文獻：
1. The simulations suggested that all missense SNPs considered as convergent deleterious caused some kind of structural change to the uroguanylin peptide. Additionally, four of these SNPs were also shown to cause modifications in peptide flexibility, possibly resulting in functional changes. PMID: 27620667
2. Uroguanylin concentrations are increased in patients with chronic renal failure, nephrotic syndrome, or those on dialysis. PMID: 15780094
3. The occurrences of the C-A haplotype (rs883062-rs1047047) and the C-A-G haplotype (rs883062-rs1047047-rs2297566) were significantly higher in the essential hypertension group than in the controls. PMID: 18037771
亞細胞定位：

Secreted.
蛋白家族：

Guanylin family
組織特異性：

Stomach and intestine.
數據庫鏈接：

HGNC: 4683

OMIM: 601271

KEGG: hsa:2981

STRING: 9606.ENSP00000361662

UniGene: Hs.32966