Human glypican 6 (GPC6) ELISA kit

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中文名稱：

人磷脂酰肌醇蛋白聚糖6(GPC6)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL009708HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人磷脂酰肌醇蛋白聚糖6(GPC6)酶聯(lián)免疫試劑盒（CSB-EL009708HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的GPC6含量。GPC6 是一個(gè)靶點(diǎn)。它在人體生理和病理過程中有重要作用。研究發(fā)現(xiàn)其參與細(xì)胞信號(hào)傳導(dǎo)等機(jī)制。圍繞它探究疾病發(fā)生發(fā)展機(jī)制、尋找有效治療靶點(diǎn)是當(dāng)前研究方向，有望為相關(guān)疾病如特定癌癥等的診治帶來新突破。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于腫瘤生物學(xué)研究、細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)機(jī)制探索、骨骼發(fā)育相關(guān)實(shí)驗(yàn)等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

A Kit; Glypican 6 [Precursor] ELISA Kit; Glypican proteoglycan 6 ELISA Kit; Gpc6 ELISA Kit; GPC6_HUMAN ELISA Kit; MGC126288 ELISA Kit; OMIMD1 ELISA Kit; PRO705 ELISA Kit; Secreted glypican 6 ELISA Kit; Secreted glypican-6 ELISA Kit; UNQ369 ELISA Kit
縮寫：

GPC6
Uniprot No.：

Q9Y625
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	107
	Range %	103-110
1:8	Average %	110
	Range %	104-114

回收率：

The recovery of human GPC6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.443	2.403	2.423	2.271
10	1.692	1.702	1.697	1.545
5	1.218	1.268	1.243	1.091
2.5	0.854	0.865	0.860	0.708
1.25	0.602	0.612	0.607	0.455
0.625	0.413	0.403	0.408	0.256
0.312	0.274	0.268	0.271	0.119
0	0.153	0.150	0.152

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts. I GONZALEZ,/,2024
Elevated plasma glypicans are associated with organ failure in patients with infection Fisher J, et al,Intensive Care Medicine Experimental,2019

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功能：

Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling.
基因功能參考文獻(xiàn)：
1. Thus, GPC6 and TMEM132D may serve as predictors of CD8+ T-lymphocyte infiltration and as favorable prognostic markers in early stage ovarian cancer with important consequences for diagnosis. PMID: 26448945
2. Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line. PMID: 24804215
3. GPC6 promotes invasive migration by inhibition of canonical beta-catenin and Wnt signalling, and up-regulation of non-canonical Wnt5A signalling leading to the activation of JNK and p38 MAPK. PMID: 21871017
4. a reduction in GPC6 mRNA in retinoblastoma is associated with the non-random allelic loss at 13q31 that could contribute to RB development. PMID: 19726429
5. Mutations in the heparan-sulfate proteoglycan glypican 6 impair endochondral ossification and cause recessive omodysplasia PMID: 19481194
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相關(guān)疾病：

Omodysplasia 1 (OMOD1)
亞細(xì)胞定位：

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-6]: Secreted, extracellular space.
蛋白家族：

Glypican family
組織特異性：

Widely expressed. High expression in fetal kidney and lung and lower expressions in fetal liver and brain. In adult tissues, very abundant in ovary, high levels also observed in liver, kidney, small intestine and colon. Not detected in peripheral blood le
數(shù)據(jù)庫鏈接：

HGNC: 4454

OMIM: 258315

KEGG: hsa:10082

STRING: 9606.ENSP00000366246

UniGene: Hs.444329

Human glypican 6 (GPC6) ELISA kit

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